Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 190704..191506 Replicon plasmid pWSM1455_1
Accession NZ_CP088091
Organism Rhizobium laguerreae strain WSM1455

Toxin (Protein)


Gene name TacT3 Uniprot ID A0A1S9GBD6
Locus tag RlegWSM1455_RS24885 Protein ID WP_025396848.1
Coordinates 190704..191231 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name TacA3 Uniprot ID W0IPV9
Locus tag RlegWSM1455_RS24890 Protein ID WP_025396849.1
Coordinates 191228..191506 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RlegWSM1455_RS24875 (RlegWSM1455_24875) 187324..188448 - 1125 WP_231285631.1 DNA repair exonuclease -
RlegWSM1455_RS24880 (RlegWSM1455_24880) 188766..190412 - 1647 WP_025396847.1 acetolactate synthase large subunit -
RlegWSM1455_RS24885 (RlegWSM1455_24885) 190704..191231 - 528 WP_025396848.1 GNAT family N-acetyltransferase Toxin
RlegWSM1455_RS24890 (RlegWSM1455_24890) 191228..191506 - 279 WP_025396849.1 DUF1778 domain-containing protein Antitoxin
RlegWSM1455_RS24895 (RlegWSM1455_24895) 191727..192605 - 879 WP_131641114.1 DUF6030 family protein -
RlegWSM1455_RS24900 (RlegWSM1455_24900) 193021..193743 - 723 WP_170269210.1 ribonuclease activity regulator RraA -
RlegWSM1455_RS24905 (RlegWSM1455_24905) 193814..194599 - 786 WP_026158174.1 IclR family transcriptional regulator -
RlegWSM1455_RS24910 (RlegWSM1455_24910) 194814..196334 + 1521 WP_231285632.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB 1..1064136 1064136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Vibrio phage ICP1, Orf50

(12-87)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 18773.72 Da        Isoelectric Point: 9.5507

>T226607 WP_025396848.1 NZ_CP088091:c191231-190704 [Rhizobium laguerreae]
VTLPVWHEEPIAKSHDRASFDCGDGAMNEFIRRFARQSHEQNAAKTFCAIDKAQPDRILGFYTVAPSAVTHEAVPPKLTR
GLARHEVAGFKLARLATDIKVAGKGLGGQLIAAAALRCLRLASEGGGILLIIDAKSERAAHWYASYGAEPLQGKPLTLVM
PLATFAADFKAKGLL

Download         Length: 528 bp

>T226607 NZ_CP122573:c5210417-5210034 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10171.84 Da        Isoelectric Point: 8.6184

>AT226607 WP_025396849.1 NZ_CP088091:c191506-191228 [Rhizobium laguerreae]
MPRTADNKTERYQLRIPPKQKAIIARAAALSNKDMADFILDKIVPEAEAVIQAAEVETVSSRDFIRILDLLEHPPKPNAK
LRAAISALPDAL

Download         Length: 279 bp

>AT226607 NZ_CP122573:c5210659-5210417 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1S9GBD6

Antitoxin

Source ID Structure
AlphaFold DB A0A1S9GBB9

References