Detailed information of TA system TA226598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 3480482..3481016
• Replicon: chromosome
• Accession: NZ_CP088087
• Organism: Bradyrhizobium arachidis strain CB756

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A1I7LMG3
• Locus tag: BaraCB756_RS16080
• Protein ID: WP_027558766.1
• Coordinates: 3480482..3480742 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: BaraCB756_RS16085
• Protein ID: WP_028146350.1
• Coordinates: 3480723..3481016 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BaraCB756_RS16055 (BaraCB756_16055)	3475917..3476333	+	417	WP_027558761.1	MucR family transcriptional regulator	-
BaraCB756_RS16060 (BaraCB756_16060)	3476455..3476883	-	429	WP_027558762.1	SufE family protein	-
BaraCB756_RS16065 (BaraCB756_16065)	3476952..3477404	-	453	WP_027558763.1	DUF5330 domain-containing protein	-
BaraCB756_RS16070 (BaraCB756_16070)	3477813..3479669	+	1857	WP_027558764.1	PAS domain-containing sensor histidine kinase	-
BaraCB756_RS16075 (BaraCB756_16075)	3479662..3480372	+	711	WP_027558765.1	peptidoglycan-binding domain-containing protein	-
BaraCB756_RS16080 (BaraCB756_16080)	3480482..3480742	+	261	WP_027558766.1	BrnT family toxin	Toxin
BaraCB756_RS16085 (BaraCB756_16085)	3480723..3481016	+	294	WP_028146350.1	BrnA antitoxin family protein	Antitoxin
BaraCB756_RS16090 (BaraCB756_16090)	3481020..3481364	+	345	WP_027558768.1	DUF1491 family protein	-
BaraCB756_RS16095 (BaraCB756_16095)	3481361..3482338	-	978	WP_027558769.1	DUF2336 domain-containing protein	-
BaraCB756_RS16100 (BaraCB756_16100)	3482593..3482781	+	189	WP_027558770.1	hypothetical protein	-
BaraCB756_RS16105 (BaraCB756_16105)	3482796..3483557	+	762	WP_027558771.1	hypothetical protein	-
BaraCB756_RS16110 (BaraCB756_16110)	3483511..3484416	-	906	WP_027558772.1	LysR family transcriptional regulator	-
BaraCB756_RS16115 (BaraCB756_16115)	3484511..3485338	+	828	WP_027558773.1	MBL fold metallo-hydrolase	-
BaraCB756_RS16120 (BaraCB756_16120)	3485375..3485593	-	219	WP_231164311.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (20-87)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9820.28 Da
• Isoelectric Point: 10.4463

>T226598 WP_027558766.1 NZ_CP088087:3480482-3480742 [Bradyrhizobium arachidis]
VKIVWDEPKRRANLDKHGMDFADLNEAFFDNALVLATYGNRWRAIGINIRGVVSVIFATRGAEGVSIVSMRPASKKERRL
YEENRL

Nucleotide

• Length: 261 bp

>T226598 NZ_CP122573:351316-351663 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10912.58 Da
• Isoelectric Point: 11.0421

>AT226598 WP_028146350.1 NZ_CP088087:3480723-3481016 [Bradyrhizobium arachidis]
MKKTVSKKGYTARDLRAVSDNPEWTKDDFAKAKTFDEVFPGMRKGRGPNKAPTKRQVTLRLSPVVVDYFKAEGPGWQSRI
DEALVKVVKRKRSSATG

Nucleotide

• Length: 294 bp

>AT226598 NZ_CP122573:351663-351962 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A1I7LMG3