Detailed information of TA system TA226597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 2673724..2674427
• Replicon: chromosome
• Accession: NZ_CP088087
• Organism: Bradyrhizobium arachidis strain CB756

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BaraCB756_RS12355
• Protein ID: WP_027564307.1
• Coordinates: 2673724..2674146 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BaraCB756_RS12360
• Protein ID: WP_027564308.1
• Coordinates: 2674143..2674427 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BaraCB756_RS12325 (BaraCB756_12325)	2669775..2670185	+	411	WP_027564302.1	acyl-CoA thioesterase	-
BaraCB756_RS12330 (BaraCB756_12330)	2670377..2670568	+	192	WP_024341426.1	hypothetical protein	-
BaraCB756_RS12335 (BaraCB756_12335)	2670713..2671105	-	393	WP_027564303.1	nuclear transport factor 2 family protein	-
BaraCB756_RS12340 (BaraCB756_12340)	2671477..2671929	-	453	WP_027564304.1	nuclear transport factor 2 family protein	-
BaraCB756_RS12345 (BaraCB756_12345)	2672084..2672770	+	687	WP_027564305.1	HAD-IA family hydrolase	-
BaraCB756_RS12350 (BaraCB756_12350)	2672874..2673719	+	846	WP_027564306.1	DeoR/GlpR family DNA-binding transcription regulator	-
BaraCB756_RS12355 (BaraCB756_12355)	2673724..2674146	-	423	WP_027564307.1	type II toxin-antitoxin system VapC family toxin	Toxin
BaraCB756_RS12360 (BaraCB756_12360)	2674143..2674427	-	285	WP_027564308.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BaraCB756_RS12365 (BaraCB756_12365)	2674659..2676209	+	1551	WP_027564309.1	glycerol-3-phosphate dehydrogenase	-
BaraCB756_RS12370 (BaraCB756_12370)	2676206..2677285	+	1080	WP_027564310.1	ABC transporter ATP-binding protein	-
BaraCB756_RS12375 (BaraCB756_12375)	2677297..2678382	+	1086	WP_027564311.1	ABC transporter ATP-binding protein	-
BaraCB756_RS12380 (BaraCB756_12380)	2678382..2679356	+	975	WP_027564312.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (15-77)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15421.68 Da
• Isoelectric Point: 6.4737

>T226597 WP_027564307.1 NZ_CP088087:c2674146-2673724 [Bradyrhizobium arachidis]
VNLLLDTNVLSEVRRPAPSPKVLAWLDTIDEDRTFISVASIAELRRGVASLEDGRRRTALAAWLTHDLPARFAERVLPID
HAVAERWGDLMAQSRRSGVVLSVMDGFFAATALANSLTLVTRNVKDFAPFGLSLLNPWDA

Nucleotide

• Length: 423 bp

>T226597 NZ_CP122573:324568-324936 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10254.59 Da
• Isoelectric Point: 5.6921

>AT226597 WP_027564308.1 NZ_CP088087:c2674427-2674143 [Bradyrhizobium arachidis]
MVDSNPAPATLPTDDTWTLANAKARLSEVIDRAQTGPQVITRHGKPNAVVVSAEEWARKVARKGTLAEFLLASPLRGADL
ELDRVHDTPRDEMP

Nucleotide

• Length: 285 bp

>AT226597 NZ_CP122573:324350-324571 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA