Detailed information of TA system TA226596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3794322..3794941
• Replicon: chromosome
• Accession: NZ_CP088086
• Organism: Bradyrhizobium canariense strain WSM471

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H5Y864
• Locus tag: BcanWSM471_RS17210
• Protein ID: WP_007609137.1
• Coordinates: 3794322..3794714 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BcanWSM471_RS17215
• Protein ID: WP_035974028.1
• Coordinates: 3794711..3794941 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BcanWSM471_RS17195 (BcanWSM471_17210)	3790807..3791874	+	1068	WP_035974952.1	Ppx/GppA phosphatase family protein	-
BcanWSM471_RS17200 (BcanWSM471_17215)	3791876..3792565	+	690	WP_007609132.1	RlmE family RNA methyltransferase	-
BcanWSM471_RS17205 (BcanWSM471_17220)	3792670..3794160	-	1491	WP_007609135.1	MFS transporter	-
BcanWSM471_RS17210 (BcanWSM471_17225)	3794322..3794714	-	393	WP_007609137.1	type II toxin-antitoxin system VapC family toxin	Toxin
BcanWSM471_RS17215 (BcanWSM471_17230)	3794711..3794941	-	231	WP_035974028.1	hypothetical protein	Antitoxin
BcanWSM471_RS17220 (BcanWSM471_17235)	3795205..3796695	+	1491	WP_007609139.1	IMP dehydrogenase	-
BcanWSM471_RS17225 (BcanWSM471_17240)	3796816..3797838	+	1023	WP_007609140.1	NADP-dependent oxidoreductase	-
BcanWSM471_RS17230 (BcanWSM471_17245)	3797994..3798290	+	297	WP_007609142.1	hypothetical protein	-
BcanWSM471_RS17235 (BcanWSM471_17250)	3798307..3799149	-	843	WP_007609144.1	metallophosphoesterase	-
BcanWSM471_RS17240 (BcanWSM471_17255)	3799220..3799879	-	660	WP_007609145.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (3-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14570.79 Da
• Isoelectric Point: 6.7597

>T226596 WP_007609137.1 NZ_CP088086:c3794714-3794322 [Bradyrhizobium canariense]
VIVVDASALLEVLLRTPAAATVEQRLFAPHQTLHAPHLLDVEVAQVIQRYAANREINVERGRMALADLADLSLRRYPHDF
LLPRIWELRNNLTAYDATYVALAEALDTPLLTRDRRLAAAPGHHAQIQLV

Nucleotide

• Length: 393 bp

>T226596 NZ_CP122572:c2630-2316 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGAGATTTACAACGCCTCCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGCCGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CATTTCTGCCGCTGGAATATCAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCGGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8630.98 Da
• Isoelectric Point: 11.5079

>AT226596 WP_035974028.1 NZ_CP088086:c3794941-3794711 [Bradyrhizobium canariense]
MTTMIQIRNVPDALHRRLKSRAALAGMSLSDYLLSEIRQVAERPTLDELRARLRSRTETMPSIAPAEAVRAERDRA

Nucleotide

• Length: 231 bp

>AT226596 NZ_CP122572:c2896-2618 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AAGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCAGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Toxin

• AlphaFold DB: H5Y864