Detailed information of TA system TA226595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1220235..1220834
• Replicon: chromosome
• Accession: NZ_CP088086
• Organism: Bradyrhizobium canariense strain WSM471

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: H5YDN3
• Locus tag: BcanWSM471_RS05710
• Protein ID: WP_007605287.1
• Coordinates: 1220538..1220834 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H5YDN2
• Locus tag: BcanWSM471_RS05705
• Protein ID: WP_007605286.1
• Coordinates: 1220235..1220534 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BcanWSM471_RS05685 (BcanWSM471_05685)	1216138..1216569	-	432	WP_007605278.1	thioesterase family protein	-
BcanWSM471_RS05690 (BcanWSM471_05690)	1216580..1217341	-	762	WP_007605280.1	SDR family NAD(P)-dependent oxidoreductase	-
BcanWSM471_RS05695 (BcanWSM471_05695)	1217362..1219248	-	1887	WP_007605283.1	feruloyl-CoA synthase	-
BcanWSM471_RS05700 (BcanWSM471_05700)	1219245..1220054	-	810	WP_007605285.1	crotonase/enoyl-CoA hydratase family protein	-
BcanWSM471_RS05705 (BcanWSM471_05705)	1220235..1220534	-	300	WP_007605286.1	putative addiction module antidote protein	Antitoxin
BcanWSM471_RS05710 (BcanWSM471_05710)	1220538..1220834	-	297	WP_007605287.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BcanWSM471_RS05715 (BcanWSM471_05715)	1220902..1221363	-	462	WP_007605288.1	DUF3237 domain-containing protein	-
BcanWSM471_RS05720 (BcanWSM471_05720)	1221376..1222695	-	1320	WP_007599671.1	TRAP transporter permease	-
BcanWSM471_RS05725 (BcanWSM471_05725)	1222698..1223207	-	510	WP_007605289.1	TRAP transporter small permease	-
BcanWSM471_RS05730 (BcanWSM471_05730)	1223430..1224446	-	1017	WP_007605290.1	TRAP transporter substrate-binding protein	-
BcanWSM471_RS05735 (BcanWSM471_05735)	1224661..1225830	+	1170	WP_007605291.1	4-hydroxybenzoate 3-monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10840.53 Da
• Isoelectric Point: 8.9630

>T226595 WP_007605287.1 NZ_CP088086:c1220834-1220538 [Bradyrhizobium canariense]
MPTIKRTDEFSDWLRNLRDRRAKAKILARIDRLTLGNPGDVAPIGGGISEMRVHEGAGYRIYYVFRGEEIIVLLCGGDKG
SQPADIALAKKIASELEV

Nucleotide

• Length: 297 bp

>T226595 NZ_CP122571:c6113-5808 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10334.80 Da
• Isoelectric Point: 4.7513

>AT226595 WP_007605286.1 NZ_CP088086:c1220534-1220235 [Bradyrhizobium canariense]
MAKVKSFDAAEYLDSPEMIAAYLTEAFESDDPSAITMAIGAVARSQGMGAIAEKAGLSRENLYRSLGGEAKPEFGTVIKV
LHAMGINLVAQPQSGTKAA

Nucleotide

• Length: 300 bp

>AT226595 NZ_CP122571:c6333-6115 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: H5YDN3

Antitoxin

• AlphaFold DB: H5YDN2