Detailed information of TA system TA226585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3988528..3989168
• Replicon: chromosome
• Accession: NZ_CP088019
• Organism: Pectobacterium carotovorum strain 25.1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LQF52_RS18075
• Protein ID: WP_010295080.1
• Coordinates: 3988528..3988944 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A419ARS9
• Locus tag: LQF52_RS18080
• Protein ID: WP_039545679.1
• Coordinates: 3988941..3989168 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQF52_RS18055 (LQF52_18055)	3984064..3985050	-	987	WP_039545673.1	quinone oxidoreductase	-
LQF52_RS18060 (LQF52_18060)	3985298..3986704	+	1407	WP_010279223.1	replicative DNA helicase	-
LQF52_RS18065 (LQF52_18065)	3986746..3987828	+	1083	WP_039545675.1	alanine racemase	-
LQF52_RS18070 (LQF52_18070)	3987821..3988459	+	639	WP_039545677.1	hypothetical protein	-
LQF52_RS18075 (LQF52_18075)	3988528..3988944	-	417	WP_010295080.1	type II toxin-antitoxin system VapC family toxin	Toxin
LQF52_RS18080 (LQF52_18080)	3988941..3989168	-	228	WP_039545679.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LQF52_RS18085 (LQF52_18085)	3989899..3990378	+	480	WP_039545680.1	Lrp/AsnC family transcriptional regulator	-
LQF52_RS18090 (LQF52_18090)	3990628..3991242	+	615	WP_010295088.1	YitT family protein	-
LQF52_RS18095 (LQF52_18095)	3991307..3992500	+	1194	WP_039530885.1	amino acid aminotransferase	-
LQF52_RS18100 (LQF52_18100)	3992669..3993166	+	498	WP_231051747.1	M48 family metallopeptidase	-
LQF52_RS18105 (LQF52_18105)	3993547..3993783	+	237	Protein_3547	type VI secretion system tube protein TssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15216.77 Da
• Isoelectric Point: 8.7720

>T226585 WP_010295080.1 NZ_CP088019:c3988944-3988528 [Pectobacterium carotovorum]
VSRLFMLDTNICSFIMREHPAEVIEKLQQCTLRRDRIVVSAITYAEMRFGAVGKKASPRHMQLVDAFCRRLDAVLPWDSA
AVDATTKIKIALAAAGTPIGPNDTAIAGHAISAKAILVTNNLREFERVPGLTLEDWVK

Nucleotide

• Length: 417 bp

>T226585 NZ_CP122562:2594153-2594578 [Auritidibacter ignavus]
GTGATCATCCTCGACACAAACGTGATCTCGGAGATCTTTCGACCCTTGCCCGAGCCACGCGTAGCGGACTGGCTCACGTC
CCTGGAGGGTGACGTAGCCATCACGTCCGTCACGCTCGCAGAACTGCTGGCAGGTTTGCGGCGGTTGCCGGATGGTCGGC
GCAGAGATGCGCTGACCAGACGAATTGACGTGGCGCTCGCACCGTACAGGGGCGGCCGCGCTGTGTTGCCGTTCGATGAC
GTGGCCGCCGACCGCTATGCCGACGTGCTCGTGGCTCGAGAGCGCGCGGGAGCGCCGATCAGTACCGCCGACGCGCAGAT
CGCCGCGATCTGCCTGGCGCACGGAGCAACCTGCGCTACGCGCAATGTGAAGGACTTCCAGCACACCGGCGTAGAACTGG
TGGATCCGTGGAAGGTCGATGCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8880.02 Da
• Isoelectric Point: 4.5098

>AT226585 WP_039545679.1 NZ_CP088019:c3989168-3988941 [Pectobacterium carotovorum]
MRIVSVFKNGNNRAIRLPRDMDFEGVNELEIVREGDTIMLRPVKPNWHSFLQEDRADADFMTERQDVVEEGRFEL

Nucleotide

• Length: 228 bp

>AT226585 NZ_CP122562:2593923-2594156 [Auritidibacter ignavus]
ATGTCATCCATCATCGTTCGCGGTCTCGACGACGCCGTGAAGCAGCAACTCGCTGCACAGGCGAAGGAGCACGGTCGGTC
GATGGAAGCCGAAGTTCGCGCCATCCTGACCAAAGCTGCACGCCGGCCGCACATAGGAATCGCTCTGCTGGCAGCCGCGC
AGGATGTCGGGGGAGTCGAGGAGCTACCGATCCCGGTGCGCGATGACGTCGCGCGGGCGGTGGACTTCGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A419ARS9