Detailed information of TA system TA226581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1313543..1314168
• Replicon: chromosome
• Accession: NZ_CP088019
• Organism: Pectobacterium carotovorum strain 25.1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: C6DB72
• Locus tag: LQF52_RS05835
• Protein ID: WP_005976087.1
• Coordinates: 1313543..1313746 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: LQF52_RS05840
• Protein ID: WP_119880753.1
• Coordinates: 1313800..1314168 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQF52_RS05805 (LQF52_05805)	1309000..1309338	+	339	WP_002208627.1	P-II family nitrogen regulator	-
LQF52_RS05810 (LQF52_05810)	1309378..1310664	+	1287	WP_199689758.1	ammonium transporter AmtB	-
LQF52_RS05815 (LQF52_05815)	1310801..1311664	-	864	WP_039472390.1	acyl-CoA thioesterase II	-
LQF52_RS05820 (LQF52_05820)	1311877..1312458	+	582	WP_119880754.1	YbaY family lipoprotein	-
LQF52_RS05825 (LQF52_05825)	1312478..1312798	-	321	WP_015839360.1	MGMT family protein	-
LQF52_RS05835 (LQF52_05835)	1313543..1313746	-	204	WP_005976087.1	HHA domain-containing protein	Toxin
LQF52_RS05840 (LQF52_05840)	1313800..1314168	-	369	WP_119880753.1	Hha toxicity modulator TomB	Antitoxin
LQF52_RS05845 (LQF52_05845)	1314767..1314910	-	144	WP_005976091.1	type B 50S ribosomal protein L36	-
LQF52_RS05850 (LQF52_05850)	1314929..1315177	-	249	WP_039506474.1	type B 50S ribosomal protein L31	-
LQF52_RS05855 (LQF52_05855)	1315410..1318538	-	3129	WP_231051942.1	efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8145.52 Da
• Isoelectric Point: 8.9008

>T226581 WP_005976087.1 NZ_CP088019:c1313746-1313543 [Pectobacterium carotovorum]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFFSAADHRLAELTMNKLYDKVPTAVWRYVR

Nucleotide

• Length: 204 bp

>T226581 NZ_CP122540:2019764-2019952 [Pseudomonas brenneri]
GTGCAAAGCAGGTTATTGATCAAGGAACTGCAAGAGGCGGGCTGGGTGCTGGATCGGGTTACGGGCAGCCATCATATTTT
CACCCACCGTTATAACCCGTACACGATACCGGTGCCTCATCCCAAGAAGGACCTACCCTTAGGCACCGTGCGAAGTATCA
GGCGGCGCGCCGGGCTGTTTTCGTTCTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14066.79 Da
• Isoelectric Point: 4.4787

>AT226581 WP_119880753.1 NZ_CP088019:c1314168-1313800 [Pectobacterium carotovorum]
MDEYTPKHYDIAQLRFLCENLCDESIATLGDSSHGWVNDPTSAFNLQLNELIEHIATFILTFKIKYPNESELSEQVEKYL
DDTYVLFSNYGINDAELRRWQKSKAKLFGMFSGENVCTPAKT

Nucleotide

• Length: 369 bp

>AT226581 NZ_CP122540:2019976-2020380 [Pseudomonas brenneri]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTTACCGCCTCCGGTATCCAGATCCCCGATATTCCAGGTGCGGT
GACCGCCGGGGACAGTTTTGAAGAGGCTTACAACGCCGCAGTGGAAGTGGCCCACATCATGCTGCAAGAGATGGCCAGCG
AAGGCCGTGCGATTCCCATGCCGACCTCGGTCGCGGCGCATCATGGCAACACGGAATATGCCGGGATGGGCTGGGGCATG
CTGGAGCTGGATATTTCGCCTTACCTGGGCAAGACCGAGAAGGTCAATGTGACCTTGCCAGGGTATGTGATCCAGCGCAT
TGACCGTTATGTACGCGAGCACAAAGTCAAAAGCCGTTCGTCATTCCTGGCGGATGCCGCGTTGGAGAAATTGGTGCGTT
CTTAG

Structures

Toxin

• AlphaFold DB: A0A1D7Z3F0