Detailed information of TA system TA226567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 747962..748560
• Replicon: plasmid pT136c
• Accession: NZ_CP088013
• Organism: Rhizobium sp. T136

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W6S2Q5
• Locus tag: LPB79_RS38005
• Protein ID: WP_024316374.1
• Coordinates: 748267..748560 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W6RK36
• Locus tag: LPB79_RS38000
• Protein ID: WP_024316375.1
• Coordinates: 747962..748267 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPB79_RS37980 (LPB79_37980)	743948..745309	+	1362	WP_024316379.1	mandelate racemase/muconate lactonizing enzyme family protein	-
LPB79_RS37985 (LPB79_37985)	745327..746526	+	1200	WP_024316378.1	mandelate racemase/muconate lactonizing enzyme family protein	-
LPB79_RS37990 (LPB79_37990)	746543..746704	-	162	Protein_744	Ku protein	-
LPB79_RS37995 (LPB79_37995)	746709..747733	+	1025	Protein_745	acetoacetate--CoA ligase	-
LPB79_RS38000 (LPB79_38000)	747962..748267	+	306	WP_024316375.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LPB79_RS38005 (LPB79_38005)	748267..748560	+	294	WP_024316374.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
LPB79_RS38010 (LPB79_38010)	749230..749826	-	597	WP_024316372.1	mobile mystery protein B	-
LPB79_RS38015 (LPB79_38015)	749832..750296	-	465	WP_024316371.1	mobile mystery protein A	-
LPB79_RS38020 (LPB79_38020)	750336..750749	+	414	WP_141652579.1	hypothetical protein	-
LPB79_RS38025 (LPB79_38025)	751017..751274	+	258	WP_141652578.1	multidrug effflux MFS transporter	-
LPB79_RS38030 (LPB79_38030)	751271..751390	+	120	Protein_752	transposase domain-containing protein	-
LPB79_RS38035 (LPB79_38035)	751404..751637	-	234	WP_024316369.1	DUF6429 family protein	-
LPB79_RS38040 (LPB79_38040)	751765..752026	+	262	Protein_754	hypothetical protein	-
LPB79_RS38045 (LPB79_38045)	752231..752815	-	585	WP_024316367.1	protein-tyrosine-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..757934	757934

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11144.82 Da
• Isoelectric Point: 7.3171

>T226567 WP_024316374.1 NZ_CP088013:748267-748560 [Rhizobium sp. T136]
MKLVWAQYALDDRDAIFSYIEKENPRAAAHVDEAIVRAARRLLEFPESGRPGRITGTREFVIPHTPYIAAYVVKIDRIRI
LRVLHGAQMWPTEVGDE

Nucleotide

• Length: 294 bp

>T226567 NZ_CP122517:c2430937-2430761 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.72 Da
• Isoelectric Point: 4.9306

>AT226567 WP_024316375.1 NZ_CP088013:747962-748267 [Rhizobium sp. T136]
MAANALVQTRIDADVKERATAVLENMGLTLSDAVRILLTRTANEGAVPLELINHSEAHDAWFRAKVLQALEDTRPDLDDG
DAEVRFRERRAVALRKAEVRD

Nucleotide

• Length: 306 bp

>AT226567 NZ_CP122517:c2430715-2430299 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: W6S2Q5

Antitoxin

• AlphaFold DB: W6RK36