Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 192103..193592 Replicon plasmid pT136c
Accession NZ_CP088013
Organism Rhizobium sp. T136

Toxin (Protein)


Gene name HipA1 Uniprot ID W6RI32
Locus tag LPB79_RS35235 Protein ID WP_024319223.1
Coordinates 192330..193592 (+) Length 421 a.a.

Antitoxin (Protein)


Gene name HipB1 Uniprot ID W6S3E8
Locus tag LPB79_RS35230 Protein ID WP_018859659.1
Coordinates 192103..192333 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPB79_RS35190 (LPB79_35190) 187457..187710 + 254 Protein_182 plasmid stabilization protein -
LPB79_RS35195 (LPB79_35195) 187707..188017 + 311 Protein_183 PIN domain-containing protein -
LPB79_RS35200 (LPB79_35200) 188282..188653 + 372 Protein_184 chromosome partitioning protein ParB -
LPB79_RS35205 (LPB79_35205) 188733..189164 + 432 Protein_185 IS5 family transposase -
LPB79_RS35210 (LPB79_35210) 189306..190688 + 1383 WP_037068948.1 transposase -
LPB79_RS35215 (LPB79_35215) 191224..191397 + 174 Protein_187 hypothetical protein -
LPB79_RS35220 (LPB79_35220) 191526..191732 + 207 WP_231052458.1 TraG/TraD/VirD4 family protein -
LPB79_RS35225 (LPB79_35225) 191651..191872 + 222 WP_024319222.1 hypothetical protein -
LPB79_RS35230 (LPB79_35230) 192103..192333 + 231 WP_018859659.1 helix-turn-helix transcriptional regulator Antitoxin
LPB79_RS35235 (LPB79_35235) 192330..193592 + 1263 WP_024319223.1 type II toxin-antitoxin system HipA family toxin Toxin
LPB79_RS35240 (LPB79_35240) 193867..195081 + 1215 WP_082321218.1 IS701 family transposase -
LPB79_RS35245 (LPB79_35245) 195268..195551 + 284 Protein_193 DUF1330 domain-containing protein -
LPB79_RS35250 (LPB79_35250) 195697..196473 + 777 WP_024316571.1 hypothetical protein -
LPB79_RS35255 (LPB79_35255) 196470..197111 + 642 WP_024316570.1 RES family NAD+ phosphorylase -
LPB79_RS35260 (LPB79_35260) 197223..198176 - 954 WP_024316569.1 sulfonate ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - htpB 1..757934 757934
- inside IScluster/Tn - - 188802..195081 6279


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(6-104)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 421 a.a.        Molecular weight: 45494.20 Da        Isoelectric Point: 5.9525

>T226566 WP_024319223.1 NZ_CP088013:192330-193592 [Rhizobium sp. T136]
MTVRVLNVWWDGRIVGQFTQDKHGDIGFAYTEAWLDDEKTLPLSASLPKRPERFSRRECRPFFGGLLPEESQRLVAAQAL
GVSPANDFALLDRLGGDVAGALQLLPEDQEPIGAGPIADQQTTPLDEVGIVRILDALPTRPLLAGQEGLRLSLAGAQSKV
PVVLIDGQVALPLPGQATTHILKPPIARFPGTTENEAFVMRLAAAIGLDVAPVEPRSANGRPFLLAERYDRYRNADGVVH
RIHQEDFCQALGVPPETKYASEGGPTFRDCFELLRRVSERPGPDIAKLLDAAIFNLIVGNADAHGKNFSILYDNRGPRMA
PLYDLLSTVAHPDLSPKMAMRVGKRATLAEMDAAGWQAFSKETGVGLLLVRRRVKELADATGEAITQLSEKISPLAADPV
TLNHVAALISDRAKLVALSI

Download         Length: 1263 bp

>T226566 NZ_CP122517:2335153-2336475 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGATGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8138.49 Da        Isoelectric Point: 10.5957

>AT226566 WP_018859659.1 NZ_CP088013:192103-192333 [Rhizobium sp. T136]
MAKQVRSPADIGALVRSARKEQNLRQDELAGVSGVGLRFIVDLEAGKPTAQIGKVLQVLQTLGCSIDILAPGERRK

Download         Length: 231 bp

>AT226566 NZ_CP122517:2334887-2335153 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W6RI32

Antitoxin

Source ID Structure
AlphaFold DB A0A120FDX4

References