Detailed information of TA system TA226563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1119717..1120544
• Replicon: plasmid pT136a
• Accession: NZ_CP088009
• Organism: Rhizobium sp. T136

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: W6RV67
• Locus tag: LPB79_RS05330
• Protein ID: WP_024315137.1
• Coordinates: 1120020..1120544 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: W6RN80
• Locus tag: LPB79_RS05325
• Protein ID: WP_024315136.1
• Coordinates: 1119717..1120016 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPB79_RS05305 (LPB79_05305)	1115533..1116027	+	495	WP_231052094.1	nuclear transport factor 2 family protein	-
LPB79_RS05310 (LPB79_05310)	1116151..1117182	+	1032	WP_024315133.1	LLM class flavin-dependent oxidoreductase	-
LPB79_RS05315 (LPB79_05315)	1117223..1118098	+	876	WP_029710045.1	LysR family transcriptional regulator	-
LPB79_RS05320 (LPB79_05320)	1118263..1119198	+	936	WP_024315134.1	pirin family protein	-
LPB79_RS05325 (LPB79_05325)	1119717..1120016	+	300	WP_024315136.1	DUF1778 domain-containing protein	Antitoxin
LPB79_RS05330 (LPB79_05330)	1120020..1120544	+	525	WP_024315137.1	GNAT family N-acetyltransferase	Toxin
LPB79_RS05335 (LPB79_05335)	1120612..1120848	-	237	WP_231052095.1	hypothetical protein	-
LPB79_RS05340 (LPB79_05340)	1121540..1122223	+	684	WP_024315139.1	hydrolase	-
LPB79_RS05345 (LPB79_05345)	1122293..1122568	+	276	WP_024315140.1	XapX domain-containing protein	-
LPB79_RS05350 (LPB79_05350)	1122792..1123018	-	227	Protein_1070	integrase core domain-containing protein	-
LPB79_RS05355 (LPB79_05355)	1123549..1123983	-	435	WP_037070694.1	DoxX family protein	-
LPB79_RS05360 (LPB79_05360)	1123986..1124273	-	288	WP_024315142.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	wbpZ	1..1987087	1987087

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (40-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (12-87)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 18672.46 Da
• Isoelectric Point: 7.8070

>T226563 WP_024315137.1 NZ_CP088009:1120020-1120544 [Rhizobium sp. T136]
VALSVPEPLTAGHDVSEFSCGKPTLDHWLKTRALSNQEKGFTAILVVREAGRVVGYYGLAPTAVIPSILPRSIRTEQPPD
PVPCLLLGQLATDTEWAGRGIGTGLVKHALERCVQAAVLIGGRALMVNAVDHEAAQFWQRRGFTPSKDDPLVLFRSIAAI
AASLAEARQQERGT

Nucleotide

• Length: 525 bp

>T226563 NZ_CP122517:1823105-1823356 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11163.01 Da
• Isoelectric Point: 9.7713

>AT226563 WP_024315136.1 NZ_CP088009:1119717-1120016 [Rhizobium sp. T136]
MATTVERKEYPISMRLPEADVAMIDRAATLRGRSRTDFVRDAAVRAAEEIVMEQRLIRMSPEGFADFMDVLSRPAAPVPE
MVKLAKRPAAWEPGYVAKR

Nucleotide

• Length: 300 bp

>AT226563 NZ_CP122517:1823353-1823607 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: W6RV67

Antitoxin

• AlphaFold DB: W6RN80