Detailed information of TA system TA226558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/DUF433(antitoxin)
• Location: 5329644..5330143
• Replicon: chromosome
• Accession: NZ_CP088007
• Organism: Nocardia huaxiensis strain BCHNH01

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LPY97_RS24370
• Protein ID: WP_181583546.1
• Coordinates: 5329644..5329997 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LPY97_RS24375
• Protein ID: WP_231083431.1
• Coordinates: 5329994..5330143 (-)
• Length: 50 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPY97_RS24340 (LPY97_24340)	5324811..5325986	-	1176	WP_231083426.1	hypothetical protein	-
LPY97_RS24345 (LPY97_24345)	5326097..5326717	-	621	WP_181585392.1	TetR/AcrR family transcriptional regulator	-
LPY97_RS24350 (LPY97_24350)	5326817..5327164	-	348	WP_181583549.1	SPW repeat protein	-
LPY97_RS24355 (LPY97_24355)	5327321..5328502	+	1182	WP_231083428.1	FAD/NAD(P)-binding oxidoreductase	-
LPY97_RS24360 (LPY97_24360)	5328583..5329362	+	780	WP_181585391.1	aspartate/glutamate racemase family protein	-
LPY97_RS24365 (LPY97_24365)	5329399..5329653	+	255	WP_231083430.1	hypothetical protein	-
LPY97_RS24370 (LPY97_24370)	5329644..5329997	-	354	WP_181583546.1	DUF5615 family PIN-like protein	Toxin
LPY97_RS24375 (LPY97_24375)	5329994..5330143	-	150	WP_231083431.1	DUF433 domain-containing protein	Antitoxin
LPY97_RS24380 (LPY97_24380)	5330276..5331466	-	1191	WP_181583544.1	elongation factor Tu	-
LPY97_RS24385 (LPY97_24385)	5331583..5333688	-	2106	WP_181583543.1	elongation factor G	-
LPY97_RS24390 (LPY97_24390)	5333845..5334315	-	471	WP_030517120.1	30S ribosomal protein S7	-
LPY97_RS24395 (LPY97_24395)	5334315..5334689	-	375	WP_014353064.1	30S ribosomal protein S12	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Domain of unknown function DUF5615 (1-79)

Antitoxin

• Protein of unknown function DUF433 (1-36)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12816.75 Da
• Isoelectric Point: 4.6009

>T226558 WP_181583546.1 NZ_CP088007:c5329997-5329644 [Nocardia huaxiensis]
MKFVVDAQLPPALARALAAEGHEAVHVCDLGMTSASDREVWNEALEREAVIVTKDEDFVTIGRAQPNEPVPAIVWIRIGN
CSRKALLESLLPLLPTVVELLEAGERIVEIRSPQTHS

Nucleotide

• Length: 354 bp

>T226558 NZ_CP122517:286885-287412 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 50 a.a.
• Molecular weight: 5401.04 Da
• Isoelectric Point: 3.9169

>AT226558 WP_231083431.1 NZ_CP088007:c5330143-5329994 [Nocardia huaxiensis]
VKDVLELLAAGETEDSILEDYPYLEREDIRACLAFAAAEADHPILRPAS

Nucleotide

• Length: 150 bp

>AT226558 NZ_CP122517:286612-286878 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA