Detailed information of TA system TA226556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF-MazE
• Location: 49128..49702
• Replicon: plasmid pIVK83
• Accession: NZ_CP088003
• Organism: Staphylococcus epidermidis strain IVK83

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LQF30_RS12045
• Protein ID: WP_130548381.1
• Coordinates: 49370..49702 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: LQF30_RS12040
• Protein ID: WP_199798485.1
• Coordinates: 49128..49373 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQF30_RS12010 (LQF30_11970)	45176..45569	-	394	Protein_13	IS6 family transposase	-
LQF30_RS12015 (LQF30_11975)	45694..46494	+	801	WP_130548378.1	ParA family protein	-
LQF30_RS12020 (LQF30_11980)	46494..46694	+	201	WP_130559168.1	plasmid replication-associated protein	-
LQF30_RS12025 (LQF30_11985)	46810..47045	+	236	Protein_16	hypothetical protein	-
LQF30_RS12030 (LQF30_11990)	47556..48113	-	558	WP_162158606.1	recombinase family protein	-
LQF30_RS12035 (LQF30_11995)	48633..48940	+	308	Protein_18	NAD-dependent malic enzyme	-
LQF30_RS12040 (LQF30_12000)	49128..49373	+	246	WP_199798485.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LQF30_RS12045 (LQF30_12005)	49370..49702	+	333	WP_130548381.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LQF30_RS12050 (LQF30_12010)	51013..51291	+	279	WP_142589454.1	hypothetical protein	-
LQF30_RS12055 (LQF30_12015)	51293..51676	+	384	WP_002494738.1	hypothetical protein	-
LQF30_RS12060 (LQF30_12020)	51688..52461	+	774	WP_162158607.1	relaxase MobL	-
LQF30_RS12065 (LQF30_12025)	52446..52790	-	345	WP_237615018.1	hypothetical protein	-
LQF30_RS12070 (LQF30_12030)	53255..54127	+	873	WP_130548385.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..55140	55140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12560.54 Da
• Isoelectric Point: 9.2133

>T226556 WP_130548381.1 NZ_CP088003:49370-49702 [Staphylococcus epidermidis]
MINQFDVIMIDLNPTRGKEKQKYRPCVVVSNDFVNQNSPFVWILPITNRSKRFPSDIPIETKNNSVTGIIDSIQIRSLDA
KVRNFKKIDELVDSLKTDVTNTIIAHSCIV

Nucleotide

• Length: 333 bp

>T226556 NZ_CP122516:c1330915-1330643 [Helicobacter pylori]
ATGCTGACGATTGAAACCAGTAAAAAATTTGATAAGAATCTTAAAATTCTTGTTAAAAATGGGTTTGATTTAAAGCTTTT
GTATAAAGTGGTTGAAAATTTAGCCAAAGAGCAACCCCTAGCTCCCAAATACAAAGACTACCCGTTCAAAGGCGGTTTAA
AAGATTTTAGGGAATGCCACCTAAAACCGGATTTATTGCTTGTCTATCAAATCAAAAAACCAAAAAACACCCTCTTTTTA
GTAAGACTAGGCAGTCATAGCGAGCTGTTTTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8943.92 Da
• Isoelectric Point: 4.4354

>AT226556 WP_199798485.1 NZ_CP088003:49128-49373 [Staphylococcus epidermidis]
MSKLAKVVKNGNSQAITLNKSVLNESGLNIGDSLSVEVNDGEIKFTKNNKSIIDEIHDFYRNGGYYDEEEIDFGKSVGCE
L

Nucleotide

• Length: 246 bp

>AT226556 NZ_CP122516:c1331216-1330929 [Helicobacter pylori]
ATGCCAAACACCACCAACAAAGATTACACAAAATACAGCCAAAGACAGCTTTTTAATTTTTTAAATTCTATCAAGACCAA
GCAAAAAAGAGCGTTAGAAAAATTGAAAGAAATCCAAACTCAAAAACAAAGGATTAAAAAAGCGCTCCAATTTAAAGCGC
TAAACCTAACCGAAAATGGATACACCATAGAAGAAGAGCGAGAAATCTTAGCAAGGGCTAAAGATACCAAGAACCACCTT
TGTTTTAAAAGCATAGAGGATTTTAAGAAGCATTGTGAAAACTTATAA