Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF-MazE
Location 49128..49702 Replicon plasmid pIVK83
Accession NZ_CP088003
Organism Staphylococcus epidermidis strain IVK83

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag LQF30_RS12045 Protein ID WP_130548381.1
Coordinates 49370..49702 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag LQF30_RS12040 Protein ID WP_199798485.1
Coordinates 49128..49373 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQF30_RS12010 (LQF30_11970) 45176..45569 - 394 Protein_13 IS6 family transposase -
LQF30_RS12015 (LQF30_11975) 45694..46494 + 801 WP_130548378.1 ParA family protein -
LQF30_RS12020 (LQF30_11980) 46494..46694 + 201 WP_130559168.1 plasmid replication-associated protein -
LQF30_RS12025 (LQF30_11985) 46810..47045 + 236 Protein_16 hypothetical protein -
LQF30_RS12030 (LQF30_11990) 47556..48113 - 558 WP_162158606.1 recombinase family protein -
LQF30_RS12035 (LQF30_11995) 48633..48940 + 308 Protein_18 NAD-dependent malic enzyme -
LQF30_RS12040 (LQF30_12000) 49128..49373 + 246 WP_199798485.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LQF30_RS12045 (LQF30_12005) 49370..49702 + 333 WP_130548381.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LQF30_RS12050 (LQF30_12010) 51013..51291 + 279 WP_142589454.1 hypothetical protein -
LQF30_RS12055 (LQF30_12015) 51293..51676 + 384 WP_002494738.1 hypothetical protein -
LQF30_RS12060 (LQF30_12020) 51688..52461 + 774 WP_162158607.1 relaxase MobL -
LQF30_RS12065 (LQF30_12025) 52446..52790 - 345 WP_237615018.1 hypothetical protein -
LQF30_RS12070 (LQF30_12030) 53255..54127 + 873 WP_130548385.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..55140 55140


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-103)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12560.54 Da        Isoelectric Point: 9.2133

>T226556 WP_130548381.1 NZ_CP088003:49370-49702 [Staphylococcus epidermidis]
MINQFDVIMIDLNPTRGKEKQKYRPCVVVSNDFVNQNSPFVWILPITNRSKRFPSDIPIETKNNSVTGIIDSIQIRSLDA
KVRNFKKIDELVDSLKTDVTNTIIAHSCIV

Download         Length: 333 bp

>T226556 NZ_CP122516:c1330915-1330643 [Helicobacter pylori]
ATGCTGACGATTGAAACCAGTAAAAAATTTGATAAGAATCTTAAAATTCTTGTTAAAAATGGGTTTGATTTAAAGCTTTT
GTATAAAGTGGTTGAAAATTTAGCCAAAGAGCAACCCCTAGCTCCCAAATACAAAGACTACCCGTTCAAAGGCGGTTTAA
AAGATTTTAGGGAATGCCACCTAAAACCGGATTTATTGCTTGTCTATCAAATCAAAAAACCAAAAAACACCCTCTTTTTA
GTAAGACTAGGCAGTCATAGCGAGCTGTTTTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8943.92 Da        Isoelectric Point: 4.4354

>AT226556 WP_199798485.1 NZ_CP088003:49128-49373 [Staphylococcus epidermidis]
MSKLAKVVKNGNSQAITLNKSVLNESGLNIGDSLSVEVNDGEIKFTKNNKSIIDEIHDFYRNGGYYDEEEIDFGKSVGCE
L

Download         Length: 246 bp

>AT226556 NZ_CP122516:c1331216-1330929 [Helicobacter pylori]
ATGCCAAACACCACCAACAAAGATTACACAAAATACAGCCAAAGACAGCTTTTTAATTTTTTAAATTCTATCAAGACCAA
GCAAAAAAGAGCGTTAGAAAAATTGAAAGAAATCCAAACTCAAAAACAAAGGATTAAAAAAGCGCTCCAATTTAAAGCGC
TAAACCTAACCGAAAATGGATACACCATAGAAGAAGAGCGAGAAATCTTAGCAAGGGCTAAAGATACCAAGAACCACCTT
TGTTTTAAAAGCATAGAGGATTTTAAGAAGCATTGTGAAAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References