Detailed information of TA system TA226549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2238046..2239076
• Replicon: chromosome
• Accession: NZ_CP088000
• Organism: Stenotrophomonas rhizophila strain Bl2-2

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LQE85_RS10415
• Protein ID: WP_093536471.1
• Coordinates: 2238498..2239076 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: LQE85_RS10410
• Protein ID: WP_093536731.1
• Coordinates: 2238046..2238501 (+)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQE85_RS10380 (LQE85_10380)	2234627..2234878	+	252	WP_249832528.1	hypothetical protein	-
LQE85_RS10385 (LQE85_10385)	2234981..2235478	+	498	WP_095364086.1	ferritin-like domain-containing protein	-
LQE85_RS10390 (LQE85_10390)	2235524..2236060	-	537	WP_249832529.1	hypothetical protein	-
LQE85_RS10395 (LQE85_10395)	2236106..2236441	-	336	WP_095364085.1	hypothetical protein	-
LQE85_RS10400 (LQE85_10400)	2236761..2237120	-	360	WP_095364084.1	hypothetical protein	-
LQE85_RS10405 (LQE85_10405)	2237336..2237911	+	576	WP_095364083.1	metal-dependent hydrolase	-
LQE85_RS10410 (LQE85_10410)	2238046..2238501	+	456	WP_093536731.1	helix-turn-helix domain-containing protein	Antitoxin
LQE85_RS10415 (LQE85_10415)	2238498..2239076	+	579	WP_093536471.1	PIN domain-containing protein	Toxin
LQE85_RS10420 (LQE85_10420)	2239729..2239968	-	240	WP_141236271.1	hypothetical protein	-
LQE85_RS10425 (LQE85_10425)	2240445..2240735	-	291	WP_095364082.1	hypothetical protein	-
LQE85_RS10430 (LQE85_10430)	2240854..2241348	-	495	WP_249832531.1	hypothetical protein	-
LQE85_RS10435 (LQE85_10435)	2241596..2242978	-	1383	WP_249832532.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-116)

Antitoxin

• SinI-like, DNA-binding domain (80-126)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21522.74 Da
• Isoelectric Point: 4.8924

>T226549 WP_093536471.1 NZ_CP088000:2238498-2239076 [Stenotrophomonas rhizophila]
VNSSTFTAVFDACVLYPANVRDLLMRLACTRCFRARWSGDIHDEWIRNLLANRPDLTRERLDRTAQQMNDAVEDCLVADY
APLIDGIEGLPDPGDRHVVAAAIRCGASVIVTYNLKDFPAAALERWGLEVQHPDEFVENVFDLHQAQVLGAVRNMRLALK
SPPLTIEELFNVLLKAELAQTVKCLEGYKAML

Nucleotide

• Length: 579 bp

>T226549 NZ_CP122510:c82097-81816 [Escherichia coli]
TTGATTAAGTCATTTCGCCATAAAGGGCTTGAACGATTTTTCAAGACAGGATCAACAGCAGGCATTCAAGCTAAACATGC
GATTAAGCTACGCATTCAATTGACTGCTCTTAATGCAGCTAAACGCCCGGACGATATGTCTGCGCCGGGATGGGAATTGC
ACCCACTAAAGGGGGCAGACCTTAAAGGTCACTGGGCAATATCAGTAAACGGTAACTGGCGACTTACCTTCCGATTTGAA
GGCGAAGACGCAATTCTTGTTGACTATCAGGATTATCATTGA

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 16754.18 Da
• Isoelectric Point: 5.6350

>AT226549 WP_093536731.1 NZ_CP088000:2238046-2238501 [Stenotrophomonas rhizophila]
MTPQVTLPRTQDIEAATTAQRELAAYLATGFETKLVQLVDSQGRLHPIQLPMAALQLLSEILSELADGNAVKIVPVHAEL
TTQEAADLLNVSRPYLVKLLETGKLAFHKTGKHRRIRFDDLMTYKQARDEASQSALDNLAAEAQELRMGYE

Nucleotide

• Length: 456 bp

>AT226549 NZ_CP122510:c81807-81526 [Escherichia coli]
ATGAGTCGTATGCATAATCCCGCGCATCCTGGCGCGGTTCTTCGTGAGTATCTGGGAGACATATCAGTCACAGATGCAGC
TGCCGCATTAGGCATTACTCGCACGGCGCTTTCTCGGATTCTGAACGAGAAAGCAGGGATTTCTGCTGATATGGCGCTTC
GTCTGGAATCTGCTCTGGGAACCAGTGCCGAGATGTGGACTGGTATGCAATCCCAGTATGAGCTTTGGGTCGCTTCACAA
ACCGCTCGCCCAAATATCCGCCCGTTATTTTTCCACGCTTAA