Detailed information of TA system TA226529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 888424..889019
• Replicon: chromosome
• Accession: NZ_CP087994
• Organism: Acetobacterium wieringae strain Y

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LNN31_RS04270
• Protein ID: WP_228880745.1
• Coordinates: 888424..888600 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LNN31_RS04275
• Protein ID: WP_263992987.1
• Coordinates: 888633..889019 (+)
• Length: 129 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNN31_RS04240 (LNN31_04235)	883504..885036	+	1533	WP_263992986.1	IS1182 family transposase	-
LNN31_RS04245 (LNN31_04240)	885793..886068	+	276	WP_228880751.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LNN31_RS04250 (LNN31_04245)	886102..887016	-	915	WP_070370064.1	IS3 family transposase	-
LNN31_RS04255 (LNN31_04250)	887013..887297	-	285	WP_070370063.1	transposase	-
LNN31_RS04260 (LNN31_04255)	887396..887506	+	111	Protein_814	type II toxin-antitoxin system RelE/ParE family toxin	-
LNN31_RS04265 (LNN31_04260)	887577..887861	+	285	WP_263993150.1	helix-turn-helix domain-containing protein	-
LNN31_RS04270 (LNN31_04265)	888424..888600	+	177	WP_228880745.1	type II toxin-antitoxin system HicA family toxin	Toxin
LNN31_RS04275 (LNN31_04270)	888633..889019	+	387	WP_263992987.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LNN31_RS04280 (LNN31_04275)	889071..890069	+	999	WP_263992988.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LNN31_RS04285 (LNN31_04280)	890044..890622	+	579	WP_228880737.1	hypothetical protein	-
LNN31_RS04290 (LNN31_04285)	890694..890855	+	162	WP_228880734.1	hypothetical protein	-
LNN31_RS04295 (LNN31_04290)	890867..891102	+	236	Protein_821	DUF2442 domain-containing protein	-
LNN31_RS04300 (LNN31_04295)	891268..891717	+	450	WP_228880731.1	hypothetical protein	-
LNN31_RS04305 (LNN31_04300)	891963..892547	+	585	WP_263992989.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	883504..885036	1532

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (5-102)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6630.91 Da
• Isoelectric Point: 10.6498

>T226529 WP_228880745.1 NZ_CP087994:888424-888600 [Acetobacterium wieringae]
MKDKDLLKLLEKNGWVEERIKGSHHVMKKDGKTEIIPVHGKDIPTGLQKTILKRTGLK

Nucleotide

• Length: 177 bp

>T226529 NZ_CP122507:1804541-1804795 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 129 a.a.
• Molecular weight: 14185.05 Da
• Isoelectric Point: 4.0479

>AT226529 WP_263992987.1 NZ_CP087994:888633-889019 [Acetobacterium wieringae]
MLSAYPAIFHKEDGTYWVEFPDLPGCQTVGESIGEVMEEAQEALGLYLASLIEDEEEINPASDIKGIVAEDDSFTSLVAC
DLTQYVRSAKAVKKTLTIPDWLNNMAEKNSINFSQTLQKALMEKLHIA

Nucleotide

• Length: 387 bp

>AT226529 NZ_CP122507:1804293-1804544 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA