Detailed information of TA system TA226520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 1598765..1599287
• Replicon: chromosome
• Accession: NZ_CP087992
• Organism: Xylella fastidiosa subsp. multiplex strain CFBP8433

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: LOK81_RS07200
• Protein ID: WP_004085905.1
• Coordinates: 1599000..1599287 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A060HDF9
• Locus tag: LOK81_RS07195
• Protein ID: WP_004085758.1
• Coordinates: 1598765..1599007 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOK81_RS07165 (LOK81_07110)	1594175..1594357	+	183	WP_027700467.1	hypothetical protein	-
LOK81_RS07170 (LOK81_07115)	1594641..1595567	-	927	WP_004085766.1	Grx4 family monothiol glutaredoxin	-
LOK81_RS07175 (LOK81_07120)	1595666..1596409	+	744	WP_225621865.1	polysaccharide deacetylase family protein	-
LOK81_RS07180 (LOK81_07125)	1597053..1597244	-	192	WP_021358405.1	hypothetical protein	-
LOK81_RS07185 (LOK81_07130)	1597662..1598081	-	420	WP_004085763.1	putative toxin-antitoxin system toxin component, PIN family	-
LOK81_RS07190 (LOK81_07135)	1598081..1598320	-	240	WP_004085761.1	ribbon-helix-helix domain-containing protein	-
LOK81_RS07195 (LOK81_07140)	1598765..1599007	+	243	WP_004085758.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
LOK81_RS07200 (LOK81_07145)	1599000..1599287	+	288	WP_004085905.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOK81_RS07205 (LOK81_07150)	1599375..1599941	+	567	WP_004085755.1	recombinase family protein	-
LOK81_RS07210 (LOK81_07155)	1599994..1601190	-	1197	WP_004083925.1	RNA-guided endonuclease TnpB family protein	-
LOK81_RS07215 (LOK81_07160)	1601184..1601810	-	627	WP_038230952.1	IS607 family transposase	-
LOK81_RS07220 (LOK81_07165)	1602040..1603314	-	1275	WP_012337985.1	pyridoxal phosphate-dependent aminotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1597259..1618555	21296
	-	flank	IS/Tn	-	-	1599375..1599941	566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-90)

Antitoxin

• Antitoxin ParD (1-78)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11159.64 Da
• Isoelectric Point: 5.9570

>T226520 WP_004085905.1 NZ_CP087992:1599000-1599287 [Xylella fastidiosa subsp. multiplex]
MAKSYRLTPLAEADLEEIWFYTFRHWSIEQADSYHRSLVAVFEGLAAGTKQGCPSVLPDFNKYLCGSHVVYFMDYADHLD
IIRILHQRQDTERYL

Nucleotide

• Length: 288 bp

>T226520 NZ_CP122506:c35697-35443 [Escherichia coli]
ATGAGAAAAAATAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8774.77 Da
• Isoelectric Point: 6.9881

>AT226520 WP_004085758.1 NZ_CP087992:1598765-1599007 [Xylella fastidiosa subsp. multiplex]
MSRNTSVALGPHFTSFIDAQVQGGRYGTASDVVRAGLRLLEEHETKVKALQDALNVGHQSGEPRPFDSEVFLSRMHTQHG

Nucleotide

• Length: 243 bp

>AT226520 NZ_CP122506:c35446-35204 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Antitoxin

• AlphaFold DB: A0A060HDF9