Detailed information of TA system TA226519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1325107..1325718
• Replicon: chromosome
• Accession: NZ_CP087992
• Organism: Xylella fastidiosa subsp. multiplex strain CFBP8433

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A060HHU8
• Locus tag: LOK81_RS05910
• Protein ID: WP_024749166.1
• Coordinates: 1325107..1325406 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q879U0
• Locus tag: LOK81_RS05915
• Protein ID: WP_004085003.1
• Coordinates: 1325410..1325718 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOK81_RS05870 (LOK81_05820)	1320221..1321366	-	1146	WP_169709940.1	baseplate J/gp47 family protein	-
LOK81_RS05875 (LOK81_05825)	1321469..1321765	+	297	WP_004085590.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LOK81_RS05880 (LOK81_05830)	1321768..1322061	+	294	WP_004085592.1	putative addiction module antidote protein	-
LOK81_RS05885 (LOK81_05835)	1322117..1322470	-	354	WP_004084998.1	hypothetical protein	-
LOK81_RS05890 (LOK81_05840)	1322467..1323108	-	642	WP_140189635.1	Gp138 family membrane-puncturing spike protein	-
LOK81_RS05895 (LOK81_05845)	1323105..1323935	-	831	WP_004085594.1	hypothetical protein	-
LOK81_RS05900 (LOK81_05850)	1323932..1324249	-	318	WP_012382585.1	hypothetical protein	-
LOK81_RS05905 (LOK81_05855)	1324249..1325019	-	771	WP_272696003.1	hypothetical protein	-
LOK81_RS05910 (LOK81_05860)	1325107..1325406	+	300	WP_024749166.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOK81_RS05915 (LOK81_05865)	1325410..1325718	+	309	WP_004085003.1	putative addiction module antidote protein	Antitoxin
LOK81_RS05920 (LOK81_05870)	1325792..1327564	-	1773	WP_272696002.1	hypothetical protein	-
LOK81_RS05925 (LOK81_05875)	1327638..1327802	-	165	WP_014607750.1	hypothetical protein	-
LOK81_RS05930 (LOK81_05880)	1327829..1328251	-	423	WP_004085005.1	putative phage tail assembly chaperone	-
LOK81_RS05935 (LOK81_05885)	1328248..1328685	-	438	WP_004085006.1	DUF3277 family protein	-
LOK81_RS05940 (LOK81_05890)	1328695..1330191	-	1497	WP_004085007.1	DUF3383 domain-containing protein	-
LOK81_RS05945 (LOK81_05895)	1330192..1330596	-	405	WP_012337895.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1318416..1362919	44503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-98)

Antitoxin

• Uncharacterized protein HI1420 (15-100)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11364.14 Da
• Isoelectric Point: 10.3861

>T226519 WP_024749166.1 NZ_CP087992:1325107-1325406 [Xylella fastidiosa subsp. multiplex]
MTYTVKRLEGFSDWLKGLKDGLARQRLIKRLRKVQLGNFGDVQPVGEGVFEMREHFGPGWRMYYVQRGSFLIVMLGGGDK
STQQSDIRRAIELAKSLED

Nucleotide

• Length: 300 bp

>T226519 NZ_CP122505:c2706-2554 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11060.61 Da
• Isoelectric Point: 4.6535

>AT226519 WP_004085003.1 NZ_CP087992:1325410-1325718 [Xylella fastidiosa subsp. multiplex]
VTITKKINVSELPEFDAAEYLNSEEEVAAYLTAVLEENDPALLAAALGDIARSRGMSQIAKDSGITREALYKALRPGSEP
RFDTISRVCTALGIRLVAQPMH

Nucleotide

• Length: 309 bp

>AT226519 NZ_CP122505:2756-2818 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A060HHU8

Antitoxin

• AlphaFold DB: A0A060HCD4