Detailed information of TA system TA226518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1321469..1322061
• Replicon: chromosome
• Accession: NZ_CP087992
• Organism: Xylella fastidiosa subsp. multiplex strain CFBP8433

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A060HCK8
• Locus tag: LOK81_RS05875
• Protein ID: WP_004085590.1
• Coordinates: 1321469..1321765 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: LOK81_RS05880
• Protein ID: WP_004085592.1
• Coordinates: 1321768..1322061 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOK81_RS05850 (LOK81_05800)	1316639..1317607	+	969	WP_004084992.1	hypothetical protein	-
LOK81_RS05855 (LOK81_05805)	1317866..1318312	+	447	WP_004084994.1	hypothetical protein	-
LOK81_RS05860 (LOK81_05810)	1318416..1319660	-	1245	WP_272696004.1	phage tail protein	-
LOK81_RS05865 (LOK81_05815)	1319664..1320224	-	561	WP_004084996.1	DUF2612 domain-containing protein	-
LOK81_RS05870 (LOK81_05820)	1320221..1321366	-	1146	WP_169709940.1	baseplate J/gp47 family protein	-
LOK81_RS05875 (LOK81_05825)	1321469..1321765	+	297	WP_004085590.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOK81_RS05880 (LOK81_05830)	1321768..1322061	+	294	WP_004085592.1	putative addiction module antidote protein	Antitoxin
LOK81_RS05885 (LOK81_05835)	1322117..1322470	-	354	WP_004084998.1	hypothetical protein	-
LOK81_RS05890 (LOK81_05840)	1322467..1323108	-	642	WP_140189635.1	Gp138 family membrane-puncturing spike protein	-
LOK81_RS05895 (LOK81_05845)	1323105..1323935	-	831	WP_004085594.1	hypothetical protein	-
LOK81_RS05900 (LOK81_05850)	1323932..1324249	-	318	WP_012382585.1	hypothetical protein	-
LOK81_RS05905 (LOK81_05855)	1324249..1325019	-	771	WP_272696003.1	hypothetical protein	-
LOK81_RS05910 (LOK81_05860)	1325107..1325406	+	300	WP_024749166.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LOK81_RS05915 (LOK81_05865)	1325410..1325718	+	309	WP_004085003.1	putative addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1318416..1362919	44503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11169.70 Da
• Isoelectric Point: 10.0610

>T226518 WP_004085590.1 NZ_CP087992:1321469-1321765 [Xylella fastidiosa subsp. multiplex]
MIELKQTDTFRKWREKLKDARARSAIASRLDRLAFGHVGDAEPVGKGVSELRINYGPGYRVYFQRRGDTIYLLLCGGDKG
SQARDIKTALHLSEQWSE

Nucleotide

• Length: 297 bp

>T226518 NZ_CP122504:c36213-35833 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10246.59 Da
• Isoelectric Point: 5.6232

>AT226518 WP_004085592.1 NZ_CP087992:1321768-1322061 [Xylella fastidiosa subsp. multiplex]
MTEKLTSYDPAEDLTTDQAIADFMAAAFGTNDPAYVAHALGVVARAKGMTQIASQTGLSREQLYRSFSAEGNPTLRTTLA
VMKALGIELSAKPSGVH

Nucleotide

• Length: 294 bp

>AT226518 NZ_CP122504:c36434-36213 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A060HCK8