Detailed information of TA system TA226516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 475071..475657
• Replicon: chromosome
• Accession: NZ_CP087992
• Organism: Xylella fastidiosa subsp. multiplex strain CFBP8433

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q87BV5
• Locus tag: LOK81_RS02020
• Protein ID: WP_004089517.1
• Coordinates: 475403..475657 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B2I6C9
• Locus tag: LOK81_RS02015
• Protein ID: WP_004085025.1
• Coordinates: 475071..475406 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOK81_RS01985 (LOK81_01970)	471845..472378	-	534	WP_050765486.1	terminase small subunit	-
LOK81_RS01990 (LOK81_01975)	472551..472775	-	225	WP_012337656.1	hypothetical protein	-
LOK81_RS01995 (LOK81_01980)	472777..473238	-	462	WP_169709972.1	hypothetical protein	-
LOK81_RS02000 (LOK81_01985)	473228..473557	-	330	WP_004085022.1	phage holin family protein	-
LOK81_RS02005 (LOK81_01990)	473550..474050	-	501	WP_128382956.1	lysozyme	-
LOK81_RS02010 (LOK81_01995)	474150..474881	-	732	WP_169709925.1	site-specific DNA-methyltransferase	-
LOK81_RS02015 (LOK81_02000)	475071..475406	-	336	WP_004085025.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LOK81_RS02020 (LOK81_02005)	475403..475657	-	255	WP_004089517.1	type II toxin-antitoxin system HicA family toxin	Toxin
LOK81_RS02025 (LOK81_02010)	475710..476432	-	723	WP_012337661.1	hypothetical protein	-
LOK81_RS02030 (LOK81_02015)	476375..476722	-	348	WP_162010044.1	hypothetical protein	-
LOK81_RS02035 (LOK81_02020)	476910..478178	+	1269	WP_249328055.1	DNA polymerase	-
LOK81_RS02040 (LOK81_02025)	478178..478453	+	276	WP_201884958.1	VRR-NUC domain-containing protein	-
LOK81_RS02045 (LOK81_02030)	478455..478859	-	405	WP_004085029.1	hypothetical protein	-
LOK81_RS02050 (LOK81_02035)	478944..480362	+	1419	WP_169709926.1	DEAD/DEAH box helicase	-
LOK81_RS02055 (LOK81_02040)	480359..480523	+	165	WP_004086189.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	445297..484282	38985

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-80)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9639.14 Da
• Isoelectric Point: 11.2766

>T226516 WP_004089517.1 NZ_CP087992:c475657-475403 [Xylella fastidiosa subsp. multiplex]
MKRKHAKTLSAIYTRPVSANIQWRDIEALFVELGAKVEEREGSRMLVRLFGERRIFHRPHPSPNTDKGAVESIRAWLKSN
GVTP

Nucleotide

• Length: 255 bp

>T226516 NZ_CP122503:c4381761-4381588 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTATCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12324.07 Da
• Isoelectric Point: 5.6556

>AT226516 WP_004085025.1 NZ_CP087992:c475406-475071 [Xylella fastidiosa subsp. multiplex]
MKNIMTIESFKAIITYDPEIDMFRGEFVGINGGADFYAKDLKGLRREGAISLKVFLEACKEDSVEPRKCYSGKFNARISP
ELHALASEAAAAQGISLNQFVEQAIQHEVHT

Nucleotide

• Length: 336 bp

>AT226516 NZ_CP122503:c4382085-4381789 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTGAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: Q87BV5

Antitoxin

• AlphaFold DB: A0A060HAK5