Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4230664..4231283 Replicon chromosome
Accession NZ_CP087978
Organism Klebsiella variicola subsp. variicola strain ZH07

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LPW14_RS20640 Protein ID WP_002892050.1
Coordinates 4231065..4231283 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag LPW14_RS20635 Protein ID WP_008805436.1
Coordinates 4230664..4231038 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPW14_RS20625 (LPW14_20625) 4225819..4227012 + 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LPW14_RS20630 (LPW14_20630) 4227035..4230181 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit AcrB -
LPW14_RS20635 (LPW14_20635) 4230664..4231038 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
LPW14_RS20640 (LPW14_20640) 4231065..4231283 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LPW14_RS20645 (LPW14_20645) 4231442..4232008 + 567 WP_032438710.1 maltose O-acetyltransferase -
LPW14_RS20650 (LPW14_20650) 4231980..4232108 - 129 Protein_4058 hypothetical protein -
LPW14_RS20655 (LPW14_20655) 4232145..4232615 + 471 WP_008805434.1 YlaC family protein -
LPW14_RS20660 (LPW14_20660) 4232584..4234041 - 1458 WP_016160392.1 PLP-dependent aminotransferase family protein -
LPW14_RS20665 (LPW14_20665) 4234142..4234840 + 699 WP_012968815.1 GNAT family protein -
LPW14_RS20670 (LPW14_20670) 4234837..4234977 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LPW14_RS20675 (LPW14_20675) 4234977..4235240 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T226512 WP_002892050.1 NZ_CP087978:4231065-4231283 [Klebsiella variicola subsp. variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T226512 NZ_CP122503:3531269-3531487 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT226512 WP_008805436.1 NZ_CP087978:4230664-4231038 [Klebsiella variicola subsp. variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT226512 NZ_CP122503:3530869-3531243 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References