Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 1684803..1685800 Replicon chromosome
Accession NZ_CP087978
Organism Klebsiella variicola subsp. variicola strain ZH07

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag LPW14_RS08270 Protein ID WP_228961040.1
Coordinates 1685246..1685800 (-) Length 185 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A1F2MDT5
Locus tag LPW14_RS08265 Protein ID WP_012541033.1
Coordinates 1684803..1685249 (-) Length 149 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPW14_RS08250 (LPW14_08250) 1681098..1682096 + 999 WP_008804028.1 galactose/glucose ABC transporter substrate-binding protein MglB -
LPW14_RS08255 (LPW14_08255) 1682215..1683735 + 1521 WP_008804029.1 galactose/methyl galactoside ABC transporter ATP-binding protein MglA -
LPW14_RS08260 (LPW14_08260) 1683751..1684761 + 1011 WP_002912871.1 galactose/methyl galactoside ABC transporter permease MglC -
LPW14_RS08265 (LPW14_08265) 1684803..1685249 - 447 WP_012541033.1 helix-turn-helix transcriptional regulator Antitoxin
LPW14_RS08270 (LPW14_08270) 1685246..1685800 - 555 WP_228961040.1 HipA domain-containing protein Toxin
LPW14_RS08275 (LPW14_08275) 1685829..1686350 - 522 WP_025714688.1 hypothetical protein -
LPW14_RS08280 (LPW14_08280) 1686465..1687196 - 732 WP_008804033.1 outer membrane permeability protein SanA -
LPW14_RS08285 (LPW14_08285) 1687489..1688373 - 885 WP_101880685.1 cytidine deaminase -
LPW14_RS08290 (LPW14_08290) 1688502..1689197 - 696 WP_008804035.1 CidB/LrgB family autolysis modulator -
LPW14_RS08295 (LPW14_08295) 1689187..1689591 - 405 WP_004201620.1 CidA/LrgA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(2-147)

Antitoxin

Cro/C1-type helix-turn-helix domain

(78-132)


Sequences

Toxin        


Download         Length: 185 a.a.        Molecular weight: 20639.90 Da        Isoelectric Point: 5.7891

>T226500 WP_228961040.1 NZ_CP087978:c1685800-1685246 [Klebsiella variicola subsp. variicola]
MRLGMESVYSLLQKAPATILDHETTLRILIEKITSSNMVRVQNYRFDVQGFIIDWVRRDLLNIIFGNSDNHGRNTAFMKA
DNEITLAPIYDFAPMKADPEGIPRTMKWSLACESGGEYNFGAIAQALAEWIAPDTLLNALSETASQLVDLPSRLKARGVP
VQILEMPSIGFKFIPDKLARWGLL

Download         Length: 555 bp

>T226500 NZ_CP122503:203854-203961 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 149 a.a.        Molecular weight: 16396.05 Da        Isoelectric Point: 10.5417

>AT226500 WP_012541033.1 NZ_CP087978:c1685249-1684803 [Klebsiella variicola subsp. variicola]
MKTLNKKETEIQNTLARLRADNPPSLPATGVTATEKKARSEISRQRVSAGLKKVKTVDRQAVIHAIIHDIMLGAISQGEA
LKKLRVEVLGLRQDEYARLVDVSRKTLSDVENDKGNYSAEIINKIYKPFGLETGLVPISKTLISSLFK

Download         Length: 447 bp

>AT226500 NZ_CP122503:c203805-203742 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MDT5

References