Detailed information of TA system TA226497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 338783..339369
• Replicon: chromosome
• Accession: NZ_CP087978
• Organism: Klebsiella variicola subsp. variicola strain ZH07

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: LPW14_RS01545
• Protein ID: WP_002920800.1
• Coordinates: 339001..339369 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A1F2M0C2
• Locus tag: LPW14_RS01540
• Protein ID: WP_032731361.1
• Coordinates: 338783..339004 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPW14_RS01520 (LPW14_01520)	334940..335866	+	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LPW14_RS01525 (LPW14_01525)	335863..337140	+	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
LPW14_RS01530 (LPW14_01530)	337137..337904	+	768	WP_032731362.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LPW14_RS01535 (LPW14_01535)	337906..338619	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LPW14_RS01540 (LPW14_01540)	338783..339004	+	222	WP_032731361.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LPW14_RS01545 (LPW14_01545)	339001..339369	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LPW14_RS01550 (LPW14_01550)	339661..340977	+	1317	WP_086892791.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LPW14_RS01555 (LPW14_01555)	341084..341971	+	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LPW14_RS01560 (LPW14_01560)	341968..342813	+	846	WP_008806976.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LPW14_RS01565 (LPW14_01565)	342815..343885	+	1071	WP_008806977.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	335863..344622	8759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T226497 WP_002920800.1 NZ_CP087978:339001-339369 [Klebsiella variicola subsp. variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T226497 NZ_CP122500:90802-91218 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8165.18 Da
• Isoelectric Point: 4.7769

>AT226497 WP_032731361.1 NZ_CP087978:338783-339004 [Klebsiella variicola subsp. variicola]
MRTVNYSEARQNLADVLESAVTGIPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT226497 NZ_CP122500:90575-90805 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A1F2M0C2