226491

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1233632..1234549 Replicon chromosome
Accession NZ_CP087957
Organism Bacillus velezensis strain B-001

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A6A8LGT8
Locus tag LOC77_RS06510 Protein ID WP_003154806.1
Coordinates 1233803..1234549 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag LOC77_RS06505 Protein ID WP_003154807.1
Coordinates 1233632..1233802 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOC77_RS06465 (1228863) 1228863..1230485 + 1623 WP_046559520.1 pyocin knob domain-containing protein -
LOC77_RS06470 (1230498) 1230498..1230869 + 372 WP_143255832.1 XkdW family protein -
LOC77_RS06475 (1230875) 1230875..1231072 + 198 WP_044053582.1 XkdX family protein -
LOC77_RS06480 (1231129) 1231129..1231890 + 762 WP_033575307.1 hypothetical protein -
LOC77_RS06485 (1231942) 1231942..1232205 + 264 WP_003154815.1 hemolysin XhlA family protein -
LOC77_RS06490 (1232219) 1232219..1232482 + 264 WP_003154813.1 phage holin -
LOC77_RS06495 (1232496) 1232496..1233374 + 879 WP_014304858.1 N-acetylmuramoyl-L-alanine amidase -
LOC77_RS06500 (1233410) 1233410..1233535 - 126 WP_003154809.1 hypothetical protein -
LOC77_RS06505 (1233632) 1233632..1233802 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
LOC77_RS06510 (1233803) 1233803..1234549 - 747 WP_003154806.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
LOC77_RS06515 (1234654) 1234654..1235652 - 999 WP_003154805.1 inorganic phosphate transporter -
LOC77_RS06520 (1235665) 1235665..1236282 - 618 WP_003154804.1 DUF47 domain-containing protein -
LOC77_RS06525 (1236568) 1236568..1237884 - 1317 WP_003154801.1 amino acid permease -
LOC77_RS06530 (1238207) 1238207..1239157 + 951 WP_015388352.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29034.50 Da        Isoelectric Point: 4.6947

>T226491 WP_003154806.1 NZ_CP087957:c1234549-1233803 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T226491 NZ_CP122499:2714339-2714446 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT226491 WP_003154807.1 NZ_CP087957:c1233802-1233632 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT226491 NZ_CP122499:c2714292-2714226 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A8LGT8

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References