Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 475979..476616 Replicon chromosome
Accession NZ_CP087957
Organism Bacillus velezensis strain B-001

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag LOC77_RS02475 Protein ID WP_003156187.1
Coordinates 476266..476616 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag LOC77_RS02470 Protein ID WP_003156188.1
Coordinates 475979..476260 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOC77_RS02450 (472344) 472344..472943 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
LOC77_RS02455 (473036) 473036..473401 + 366 WP_014304402.1 holo-ACP synthase -
LOC77_RS02460 (473566) 473566..474573 + 1008 WP_151295737.1 outer membrane lipoprotein carrier protein LolA -
LOC77_RS02465 (474690) 474690..475859 + 1170 WP_003156189.1 alanine racemase -
LOC77_RS02470 (475979) 475979..476260 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
LOC77_RS02475 (476266) 476266..476616 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LOC77_RS02480 (476734) 476734..477555 + 822 WP_230639750.1 STAS domain-containing protein -
LOC77_RS02485 (477560) 477560..477925 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
LOC77_RS02490 (477928) 477928..478329 + 402 WP_003156178.1 anti-sigma regulatory factor -
LOC77_RS02495 (478341) 478341..479348 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
LOC77_RS02500 (479412) 479412..479741 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
LOC77_RS02505 (479738) 479738..480220 + 483 WP_003156173.1 anti-sigma B factor RsbW -
LOC77_RS02510 (480186) 480186..480974 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
LOC77_RS02515 (480974) 480974..481576 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T226490 WP_003156187.1 NZ_CP087957:476266-476616 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T226490 NZ_CP122499:2713804-2713911 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT226490 WP_003156188.1 NZ_CP087957:475979-476260 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT226490 NZ_CP122499:c2713757-2713691 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References