Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-AbrB
Location 2792050..2792602 Replicon chromosome
Accession NZ_CP087952
Organism Desulfosarcina sp. BuS5

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag BuS5_RS13715 Protein ID WP_035265615.1
Coordinates 2792050..2792385 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag BuS5_RS13720 Protein ID WP_027354226.1
Coordinates 2792375..2792602 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BuS5_RS13700 (BuS5_02756) 2788219..2789766 - 1548 WP_035265617.1 ATP-binding protein -
BuS5_RS13705 (BuS5_02757) 2789784..2790881 - 1098 WP_274427748.1 ATP-binding protein -
BuS5_RS13710 (BuS5_02758) 2790934..2792010 - 1077 WP_274427749.1 ATP-binding protein -
BuS5_RS13715 (BuS5_02759) 2792050..2792385 - 336 WP_035265615.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BuS5_RS13720 (BuS5_02760) 2792375..2792602 - 228 WP_027354226.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BuS5_RS13725 2793445..2793738 - 294 WP_157487407.1 transposase -
BuS5_RS13730 (BuS5_02762) 2793725..2794009 + 285 WP_027354225.1 hypothetical protein -
BuS5_RS13735 (BuS5_02763) 2794026..2795492 - 1467 WP_198012269.1 M48 family metalloprotease -
BuS5_RS13740 (BuS5_02764) 2795489..2796295 - 807 WP_051374850.1 inositol monophosphatase family protein -
BuS5_RS13745 (BuS5_02765) 2796301..2796771 - 471 WP_027354223.1 universal stress protein -
BuS5_RS13750 (BuS5_02766) 2797108..2797257 + 150 WP_157487406.1 hypothetical protein -
BuS5_RS13755 (BuS5_02767) 2797233..2797418 + 186 WP_027354222.1 aldehyde dehydrogenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-106)

Antitoxin

SpoVT-AbrB domain

(6-44)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12765.92 Da        Isoelectric Point: 10.0481

>T226486 WP_035265615.1 NZ_CP087952:c2792385-2792050 [Desulfosarcina sp. BuS5]
MICSRYDVVTVLFPFIDRPKTKKRPAVVLSNKEFNKSNHTILAMITTKKEPAWPGDFQISDYTDAGLRLPCLIRFKFFTL
DNRLMQKKIGHLSENDSTQIAKHLSSYLTAC

Download         Length: 336 bp

>T226486 NZ_CP122499:1890285-1890539 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8343.43 Da        Isoelectric Point: 4.7906

>AT226486 WP_027354226.1 NZ_CP087952:c2792602-2792375 [Desulfosarcina sp. BuS5]
MQHVSKLSTKGQVTIPAELRKAIGIEPGDMVAYELQGKSVKLKRVEPFDAAYHAAVSKTLEEWNSPEDEEAFNDL

Download         Length: 228 bp

>AT226486 NZ_CP122499:1890037-1890288 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References