Detailed information of TA system TA226479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1009785..1010490
• Replicon: chromosome
• Accession: NZ_CP087952
• Organism: Desulfosarcina sp. BuS5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BuS5_RS04990
• Protein ID: WP_274428064.1
• Coordinates: 1010056..1010490 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BuS5_RS04985
• Protein ID: WP_027354895.1
• Coordinates: 1009785..1010033 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BuS5_RS04955 (BuS5_01018)	1004932..1005132	+	201	WP_027355147.1	hypothetical protein	-
BuS5_RS04960 (BuS5_01019)	1005318..1006031	+	714	WP_274428061.1	Cthe_2314 family HEPN domain-containing protein	-
BuS5_RS04965	1006126..1006266	-	141	WP_274428062.1	hypothetical protein	-
BuS5_RS04970 (BuS5_01020)	1007340..1008713	+	1374	WP_274428063.1	group II intron reverse transcriptase/maturase	-
BuS5_RS04975 (BuS5_01021)	1009027..1009287	+	261	WP_084446254.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BuS5_RS04980 (BuS5_01022)	1009271..1009615	+	345	WP_027354894.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
BuS5_RS04985 (BuS5_01023)	1009785..1010033	+	249	WP_027354895.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BuS5_RS04990 (BuS5_01024)	1010056..1010490	+	435	WP_274428064.1	type II toxin-antitoxin system VapC family toxin	Toxin
BuS5_RS04995 (BuS5_01025)	1011006..1011632	-	627	WP_027354896.1	PqiC family protein	-
BuS5_RS05000 (BuS5_01026)	1011622..1013241	-	1620	WP_027354897.1	MlaD family protein	-
BuS5_RS05005 (BuS5_01027)	1013234..1014463	-	1230	WP_027354898.1	paraquat-inducible protein A	-
BuS5_RS05010 (BuS5_01028)	1014587..1015279	-	693	WP_027354899.1	DUF3313 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (15-104)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 16482.81 Da
• Isoelectric Point: 9.9990

>T226479 WP_274428064.1 NZ_CP087952:1010056-1010490 [Desulfosarcina sp. BuS5]
MNNHPPEVLQKFKSIGVGKVGISSITVSELHYGAYKSNHIKKNINRLDEFLSPFEIFSYDENASRYYGKIRSQLEKQGNI
IGPLDMLIAAHALSNNLILITNNVKEFMRIKSLQVENWIEKQGTEQSHSRAKINPNRWDKTQGL

Nucleotide

• Length: 435 bp

>T226479 NZ_CP122499:c624025-623561 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9751.11 Da
• Isoelectric Point: 8.4299

>AT226479 WP_027354895.1 NZ_CP087952:1009785-1010033 [Desulfosarcina sp. BuS5]
MDTAKLFINGRSQAVRLPKSYRFEGKEVYVKKVSEGVLLLPKDNTIWDVWEKNLKKYDKPFMTERNQPQSQQEREGLDEI
FD

Nucleotide

• Length: 249 bp

>AT226479 NZ_CP122499:c624360-624025 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA