Detailed information of TA system TA226473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 716664..718315
• Replicon: chromosome
• Accession: NZ_CP087952
• Organism: Desulfosarcina sp. BuS5

Toxin (Protein)

• Gene name: HipA3
• Uniprot ID: -
• Locus tag: BuS5_RS03385
• Protein ID: WP_027354123.1
• Coordinates: 716664..717968 (-)
• Length: 435 a.a.

Antitoxin (Protein)

• Gene name: HipB3
• Uniprot ID: -
• Locus tag: BuS5_RS03390
• Protein ID: WP_027354124.1
• Coordinates: 717965..718315 (-)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BuS5_RS03355 (BuS5_00683)	712542..713417	+	876	WP_027354118.1	DUF350 domain-containing protein	-
BuS5_RS03360 (BuS5_00684)	713552..714358	+	807	WP_027354119.1	hypothetical protein	-
BuS5_RS03365 (BuS5_00685)	714424..714834	+	411	WP_027354120.1	hypothetical protein	-
BuS5_RS03370 (BuS5_00686)	714850..715527	+	678	WP_027354121.1	PspA/IM30 family protein	-
BuS5_RS03375 (BuS5_00687)	715585..715920	+	336	WP_051374822.1	S-adenosylmethionine decarboxylase	-
BuS5_RS03380 (BuS5_00688)	715861..716709	-	849	WP_157487404.1	TIGR02757 family protein	-
BuS5_RS03385 (BuS5_00689)	716664..717968	-	1305	WP_027354123.1	type II toxin-antitoxin system HipA family toxin	Toxin
BuS5_RS03390 (BuS5_00690)	717965..718315	-	351	WP_027354124.1	helix-turn-helix transcriptional regulator	Antitoxin
BuS5_RS03395 (BuS5_00691)	718972..719553	+	582	WP_084446021.1	DUF2092 domain-containing protein	-
BuS5_RS03400 (BuS5_00692)	719550..720128	+	579	WP_027354125.1	hypothetical protein	-
BuS5_RS03405 (BuS5_00693)	720207..721229	-	1023	WP_027354126.1	hypothetical protein	-
BuS5_RS03410 (BuS5_00694)	721361..722539	-	1179	WP_027354127.1	FprA family A-type flavoprotein	-
BuS5_RS03415 (BuS5_00695)	722616..722753	-	138	WP_232223068.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (170-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (27-68)

Sequences

Toxin

Protein

• Length: 435 a.a.
• Molecular weight: 49119.43 Da
• Isoelectric Point: 9.2873

>T226473 WP_027354123.1 NZ_CP087952:c717968-716664 [Desulfosarcina sp. BuS5]
MSQVAEVKLWERTIGALSLEEGKEVADFEYDPAFAGSGIEIAPLTMPLANRVYSFPELSRMTFRGLPGLIADSLPDRFGH
ALIDVWLATRGQPPESFNAVERLCYIGRRGMGALEFEPAIGPKARKSTRVQVDKLVELASEILTHRKNLKVSFAGKKKER
ALREILKVGTSAGGARAKAVIAWNPLTNEVRSGQIPAGEGFEYWLLKFDGVKGNKDKEIEDPKGYGVIEYTYYKMATDAG
ITMSECRLFEENNRRHFMTRRFDRLDNGEKLHMQSLCALAHYDFNMAGAYAYEQALLVIRQLGLPMSAIEEQFRRMAFNI
ISRNQDDHVKNIAFLMDKSGKWSLSPAFDITYSFNPSGKWTVSHQMTMNGKRDNFRMEDFKACAMVASMKRGRAETIVNE
VLKTVSRWRDYADEAGVSPLWRDGIQQTLRLKIL

Nucleotide

• Length: 1305 bp

>T226473 NZ_CP122496:c4010779-4010429 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCAGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGAAATAA

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 13221.33 Da
• Isoelectric Point: 10.9210

>AT226473 WP_027354124.1 NZ_CP087952:c718315-717965 [Desulfosarcina sp. BuS5]
MFNFKTMTDDAVLEELGRRLERRRIDLKLTQAKLAEQAGVSKRTLERMEAGAVTQTRNLIRILRMLDLLQGFEQLIPVTG
PSPMDLLKFKGKERKRASSSSAVKQSPKTWSWGDDS

Nucleotide

• Length: 351 bp

>AT226473 NZ_CP122496:c4011024-4010773 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCTACCCCTGCGGGTGAC
GAAATATGGTAA