Detailed information of TA system TA226449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3207686..3208262
• Replicon: chromosome
• Accession: NZ_CP087880
• Organism: Pseudocitrobacter corydidari isolate G163CM

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: G163CM_RS14960
• Protein ID: WP_231825491.1
• Coordinates: 3207975..3208262 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: G163CM_RS14955
• Protein ID: WP_231825490.1
• Coordinates: 3207686..3207988 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G163CM_RS14935 (3203252)	3203252..3203764	+	513	WP_231825488.1	4-hydroxyphenylacetate 3-monooxygenase reductase subunit	-
G163CM_RS14940 (3204101)	3204101..3206251	+	2151	WP_015966065.1	carbon starvation CstA family protein	-
G163CM_RS14945 (3206480)	3206480..3206683	+	204	WP_015966066.1	YbdD/YjiX family protein	-
G163CM_RS14950 (3206695)	3206695..3207651	+	957	WP_231825489.1	GTPase	-
G163CM_RS14955 (3207686)	3207686..3207988	-	303	WP_231825490.1	BrnA antitoxin family protein	Antitoxin
G163CM_RS14960 (3207975)	3207975..3208262	-	288	WP_231825491.1	BrnT family toxin	Toxin
G163CM_RS14965 (3208459)	3208459..3209625	+	1167	WP_231825492.1	restriction endonuclease	-
G163CM_RS14970 (3209689)	3209689..3211308	+	1620	WP_231825493.1	class I SAM-dependent DNA methyltransferase	-
G163CM_RS14975 (3211298)	3211298..3212677	+	1380	WP_231825494.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11243.77 Da
• Isoelectric Point: 9.7290

>T226449 WP_231825491.1 NZ_CP087880:c3208262-3207975 [Pseudocitrobacter corydidari]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPRHLSRQDRVENGEYRWQTIGLVHGIVVILVAHTIRFESGNEMIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T226449 NZ_CP122492:c4262237-4261866 [Escherichia coli]
ATGCAAACTATATCATCACACCCGACACGGGCGACTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGCGGTTACTGAC
TTGTCTGTTGTCGCAGCACTATGGTCTGACGTTGAATGACACACCGTTCAGTAATGAAACCACCATACTGGAACACATTG
ATGCTGGAGTATCGCTGTGCGATGCGGTGAACTTTCTTGTTGAAAAATATGAACTGGTAAGAATTGACTGTAATGATTGC
TCTGTCATGGAGAAGTCACCGTTCATCACCAGTATCGATATTCTACGTGCCCGGAAAGCCTCCGGTCTGATGAAACGCAA
CAGTCACAAAACTGTTACCAGGGTAACGGCAGGTCGACTTCAGGAGCTATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11447.91 Da
• Isoelectric Point: 9.5646

>AT226449 WP_231825490.1 NZ_CP087880:c3207988-3207686 [Pseudocitrobacter corydidari]
MSMVKHKRGSSSTLNSQHEAELKALANKPDEDIDYTDIPASGDEQWSDAERGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT226449 NZ_CP122492:c4262641-4262276 [Escherichia coli]
ATGAACAATCACTCAGAATCCGGTACAAAGCCGGAGAATCCCGCCTGCCAGCAGTGGGGGCTTAAATGTGCTATTACCCC
CTGTTTTGGTGCCCGCCTGGTACAGGAGGGCAACCGATTGCATTTTCTCTCTGACCGCGCAGGATTTAGCGGGGCATTTG
CCGTCGATGTTGCAATGCGCCTTGACCAGGCATTTCCGTTAATGATGAAGCAACTTGAGCTGATGCTGACCAGTGGAGAG
CTTAACCCTCGCCACCCACACTGCGTCACGCTTTACCACAATGGACTCACCTGTGAAGCCGATACACTAGGCTCCTGTGG
TTATGTCTACATTGCTATCTATCCTGAGCAAACTGAGCCGCAGTAA