Detailed information of TA system TA226446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2553902..2554521
• Replicon: chromosome
• Accession: NZ_CP087880
• Organism: Pseudocitrobacter corydidari isolate G163CM

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A5B0KGF8
• Locus tag: G163CM_RS11860
• Protein ID: WP_015965534.1
• Coordinates: 2554303..2554521 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: G163CM_RS11855
• Protein ID: WP_231825098.1
• Coordinates: 2553902..2554276 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G163CM_RS11845 (2548986)	2548986..2550179	+	1194	WP_015965531.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
G163CM_RS11850 (2550202)	2550202..2553351	+	3150	WP_015965532.1	multidrug efflux RND transporter permease subunit AcrB	-
G163CM_RS11855 (2553902)	2553902..2554276	+	375	WP_231825098.1	Hha toxicity modulator TomB	Antitoxin
G163CM_RS11860 (2554303)	2554303..2554521	+	219	WP_015965534.1	HHA domain-containing protein	Toxin
G163CM_RS11865 (2554691)	2554691..2555245	+	555	WP_015965535.1	maltose O-acetyltransferase	-
G163CM_RS11870 (2555344)	2555344..2555814	+	471	WP_231828369.1	YlaC family protein	-
G163CM_RS11875 (2555783)	2555783..2557240	-	1458	WP_231825099.1	PLP-dependent aminotransferase family protein	-
G163CM_RS11880 (2557342)	2557342..2558055	+	714	WP_231825100.1	GNAT family protein	-
G163CM_RS11885 (2558052)	2558052..2558192	-	141	WP_103776587.1	type B 50S ribosomal protein L36	-
G163CM_RS11890 (2558192)	2558192..2558452	-	261	WP_015965539.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T226446 WP_015965534.1 NZ_CP087880:2554303-2554521 [Pseudocitrobacter corydidari]
MSEKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSTVWKFIR

Nucleotide

• Length: 219 bp

>T226446 NZ_CP122492:3523686-3523904 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14453.22 Da
• Isoelectric Point: 4.8989

>AT226446 WP_231825098.1 NZ_CP087880:2553902-2554276 [Pseudocitrobacter corydidari]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPSSAINLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSNYGINSEDLQKWRKSGNKLFRCFVNASRANPVSLSC

Nucleotide

• Length: 375 bp

>AT226446 NZ_CP122492:3523286-3523660 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A5B0KGF8