Detailed information of TA system TA226391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): AbkB-shpB/BrnT-BrnA
• Location: 965895..966447
• Replicon: chromosome
• Accession: NZ_CP087856
• Organism: Moraxella bovis strain SAM57987

Toxin (Protein)

• Gene name: AbkB
• Uniprot ID: A0A1T0A7D3
• Locus tag: LP115_RS05145
• Protein ID: WP_029102268.1
• Coordinates: 966169..966447 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: LP115_RS05140
• Protein ID: WP_078273473.1
• Coordinates: 965895..966182 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LP115_RS05120 (LP115_05100)	961330..962379	+	1050	WP_264676228.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
LP115_RS05125 (LP115_05105)	962458..963957	+	1500	WP_112742120.1	DUF389 domain-containing protein	-
LP115_RS05130	963986..964111	+	126	WP_264676709.1	hypothetical protein	-
LP115_RS05135 (LP115_05110)	964155..965885	-	1731	WP_078273474.1	flotillin domain-containing protein	-
LP115_RS05140 (LP115_05115)	965895..966182	-	288	WP_078273473.1	BrnA antitoxin family protein	Antitoxin
LP115_RS05145 (LP115_05120)	966169..966447	-	279	WP_029102268.1	BrnT family toxin	Toxin
LP115_RS05150 (LP115_05125)	966542..967198	-	657	WP_078273472.1	YqiJ family protein	-
LP115_RS05155 (LP115_05130)	967398..968063	-	666	WP_078273471.1	PspA/IM30 family protein	-
LP115_RS05160 (LP115_05135)	968276..968566	-	291	WP_264676229.1	hypothetical protein	-
LP115_RS05165 (LP115_05140)	968559..969074	-	516	WP_115369660.1	hypothetical protein	-
LP115_RS05170 (LP115_05145)	969222..969572	-	351	WP_078273468.1	TIGR01244 family sulfur transferase	-
LP115_RS05175 (LP115_05150)	969684..970460	+	777	WP_264676230.1	nucleotidyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-85)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (36-89)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10484.81 Da
• Isoelectric Point: 5.1084

>T226391 WP_029102268.1 NZ_CP087856:c966447-966169 [Moraxella bovis]
MIFEWDDNKNRTNKQKHGVSFESASRIFLDPFLIVTEDTSSDEVRWLGVGSVDGVVILLVVHTHTDTDGTEIIRIISARK
LSRGEIERYGYC

Nucleotide

• Length: 279 bp

>T226391 NZ_CP122455:49401-49706 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10979.71 Da
• Isoelectric Point: 7.0097

>AT226391 WP_078273473.1 NZ_CP087856:c966182-965895 [Moraxella bovis]
MAIVRKTLDDIKHTPIDKDELAQLQSMSDDSINYDDIPPLTDVQIANLRLYKPIKQQITLRLDSDIVAWLKKDGKGYQTR
ANQLLRQLMLKELTT

Nucleotide

• Length: 288 bp

>AT226391 NZ_CP122455:49181-49399 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A1T0A7D3