Detailed information of TA system TA022639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 494094..495636
• Replicon: chromosome
• Accession: NC_010334
• Organism: Shewanella halifaxensis HAW-EB4

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B0TQK1
• Locus tag: SHAL_RS02115
• Protein ID: WP_012275549.1
• Coordinates: 494094..495401 (-)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: SHAL_RS02120
• Protein ID: WP_012275550.1
• Coordinates: 495388..495636 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SHAL_RS02105	489231..490070	-	840	WP_012275547.1	5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase	-
SHAL_RS02110	490530..493730	-	3201	WP_041415798.1	DEAD/DEAH box helicase	-
SHAL_RS02115	494094..495401	-	1308	WP_012275549.1	HipA domain-containing protein	Toxin
SHAL_RS02120	495388..495636	-	249	WP_012275550.1	helix-turn-helix domain-containing protein	Antitoxin
SHAL_RS02125	496113..496976	-	864	WP_012275551.1	YicC family protein	-
SHAL_RS02130	497250..497963	+	714	WP_012275552.1	ribonuclease PH	-
SHAL_RS02135	498083..498724	+	642	WP_012157035.1	orotate phosphoribosyltransferase	-
SHAL_RS02140	499051..499710	-	660	WP_012275553.1	GTP cyclohydrolase I FolE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (146-388)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-65)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 49051.21 Da
• Isoelectric Point: 5.7840

>T22639 WP_012275549.1 NC_010334:c495401-494094 [Shewanella halifaxensis HAW-EB4]
MSGSNFHALEMHFRGMIIGSIGFDIDTEQFSIEYTKAWQENGFPISPKLPLNGQASSSQISIYLTNLLPENKGLDHLIEA
LGIAKSNTFGLIKGIGFDTSGAILYLPENTKPPVTSFTPISKEDLIKRIEDPEHWPMEIWDGKPRLSVAGVQSKINLFHT
DTEYGFGEGQLCSTHIIKFEKNSQQYLVLNEFITMTLAGLIGIQVAKVEIMPIGKYKALSVTRFDRMYDPERHIVMRRHQ
IDACQALGFSVARKYERHLGSGRDVRDIREGVSLEKLFSIAEYCANPAKAKFDMLRWTIFNLLVCNADSHGKNYSFYVTA
NGLEPTPWYDLVNVQMYPDFDQELAMAIGGEFEADKIHAYQLMTFAEECGIKQNILELTLKQLAKAIIANLDKAAKQAGE
LTDAESKYVGTYKATIQARCEYYLAQVEEMKHIEL

Nucleotide

• Length: 1308 bp

>T22639 NC_010334:c495401-494094 [Shewanella halifaxensis HAW-EB4]
ATGAGTGGTTCTAACTTTCACGCCCTAGAGATGCACTTTAGAGGCATGATTATTGGGTCGATTGGTTTTGACATAGACAC
TGAACAATTTTCTATCGAATACACTAAAGCTTGGCAGGAAAATGGTTTTCCTATTTCACCAAAGCTCCCACTTAATGGAC
AAGCTAGCAGTTCGCAAATCTCTATTTACCTCACTAATCTACTACCCGAAAATAAAGGCCTTGATCACCTTATTGAAGCC
CTAGGTATTGCCAAAAGTAATACATTTGGTTTGATCAAAGGCATTGGGTTCGATACCTCTGGTGCAATATTGTATCTGCC
TGAAAACACCAAACCACCAGTAACGTCTTTTACCCCGATTAGCAAAGAAGACCTTATCAAGCGAATCGAAGACCCAGAAC
ATTGGCCGATGGAAATCTGGGACGGAAAACCACGTCTTTCCGTTGCAGGGGTTCAATCAAAAATAAACCTATTCCACACC
GACACAGAATACGGATTTGGTGAAGGTCAGCTCTGTTCAACTCATATCATTAAATTTGAAAAAAACAGTCAGCAGTACCT
CGTACTCAACGAATTTATTACCATGACACTTGCCGGGCTCATTGGTATACAGGTAGCCAAAGTAGAGATAATGCCAATAG
GGAAATACAAAGCGTTATCCGTCACTCGCTTCGATCGCATGTACGATCCTGAGCGCCATATTGTCATGCGTCGTCATCAG
ATCGATGCATGTCAAGCTCTGGGCTTTTCCGTCGCAAGAAAGTATGAACGACATCTTGGTAGTGGTCGTGACGTACGAGA
TATTCGTGAAGGAGTAAGCCTAGAAAAGCTGTTTAGTATCGCAGAGTACTGTGCTAACCCTGCAAAAGCCAAGTTTGACA
TGCTTCGTTGGACAATCTTTAATTTACTCGTTTGCAATGCGGACTCACATGGCAAAAACTATTCATTCTATGTCACTGCT
AATGGGCTTGAACCGACCCCATGGTACGATCTTGTCAACGTGCAGATGTACCCTGATTTTGATCAAGAGCTTGCTATGGC
TATTGGAGGTGAGTTCGAGGCTGACAAGATCCACGCCTACCAATTAATGACATTTGCTGAAGAATGCGGAATTAAGCAAA
ACATACTTGAATTAACCCTCAAACAACTGGCGAAAGCAATTATCGCTAATCTGGATAAAGCGGCAAAACAAGCTGGGGAG
CTTACGGATGCAGAATCCAAGTATGTTGGAACGTACAAAGCAACCATTCAAGCACGATGTGAATACTACCTGGCTCAGGT
TGAAGAAATGAAGCACATTGAGCTATAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8974.41 Da
• Isoelectric Point: 10.1050

>AT22639 WP_012275550.1 NC_010334:c495636-495388 [Shewanella halifaxensis HAW-EB4]
MVTQLDPSTLGKLIKDRRKAANMTQEVAASFCGVTKKTLIRVEKGEDVYFSTIVKILSGLGIKLTVDTPNSKNNLGGSNE
WF

Nucleotide

• Length: 249 bp

>AT22639 NC_010334:c495636-495388 [Shewanella halifaxensis HAW-EB4]
ATGGTCACTCAACTCGATCCAAGCACCTTAGGCAAACTAATCAAAGACCGCCGAAAGGCCGCTAACATGACTCAGGAAGT
CGCCGCATCATTCTGTGGTGTTACCAAGAAGACCTTAATCAGAGTGGAAAAAGGTGAAGATGTATACTTTTCAACTATCG
TCAAAATCCTCAGTGGCCTTGGTATAAAGCTGACCGTCGATACACCTAATAGCAAAAACAACTTAGGGGGCAGCAATGAG
TGGTTCTAA

Structures

Toxin

• AlphaFold DB: B0TQK1