Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 4487996..4488584 Replicon chromosome
Accession NC_010322
Organism Pseudomonas putida GB-1

Toxin (Protein)


Gene name PumA Uniprot ID B0KS03
Locus tag PPUTGB1_RS20395 Protein ID WP_012273610.1
Coordinates 4487996..4488298 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID B0KS04
Locus tag PPUTGB1_RS20400 Protein ID WP_012273611.1
Coordinates 4488291..4488584 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPUTGB1_RS20375 4484263..4485156 - 894 WP_012273606.1 LysR family transcriptional regulator -
PPUTGB1_RS20380 4485263..4486423 + 1161 WP_012273607.1 MFS transporter -
PPUTGB1_RS20385 4486392..4487318 - 927 WP_012273608.1 DMT family transporter -
PPUTGB1_RS20390 4487424..4487819 - 396 WP_012273609.1 hypothetical protein -
PPUTGB1_RS20395 4487996..4488298 + 303 WP_012273610.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PPUTGB1_RS20400 4488291..4488584 + 294 WP_012273611.1 putative addiction module antidote protein Antitoxin
PPUTGB1_RS20405 4488674..4488946 + 273 WP_012273612.1 hypothetical protein -
PPUTGB1_RS20410 4489072..4490433 - 1362 WP_012273613.1 DEAD/DEAH box helicase -
PPUTGB1_RS20415 4490537..4491838 + 1302 WP_012273614.1 mechanosensitive ion channel family protein -
PPUTGB1_RS20420 4491855..4492268 - 414 WP_012273615.1 aminoacyl-tRNA hydrolase -
PPUTGB1_RS20425 4492334..4493269 - 936 WP_012273616.1 AEC family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4480198..4499599 19401


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(6-94)

Antitoxin

Cro/C1-type helix-turn-helix domain

(45-90)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11406.02 Da        Isoelectric Point: 10.6682

>T22638 WP_012273610.1 NC_010322:4487996-4488298 [Pseudomonas putida GB-1]
MKKIESSSFRHWVTGLRDSSARARIISRINRLMEGLPGDVSPVGHGVSELRIYYGPGYRVYFHQTGNTFVILLCGGDKSS
QQRDIKAAHQILRSWRMQND

Download         Length: 303 bp

>T22638 NC_010322:4487996-4488298 [Pseudomonas putida GB-1]
ATGAAGAAAATCGAATCCAGCAGTTTCAGACATTGGGTGACCGGCCTGCGGGACAGCAGCGCCAGAGCCCGCATCATTTC
TCGGATCAACCGGTTAATGGAGGGTTTACCTGGCGACGTATCTCCGGTTGGCCATGGCGTCAGTGAACTCAGGATTTACT
ACGGGCCTGGCTACCGGGTGTACTTCCATCAGACGGGCAACACTTTCGTCATTCTGTTATGTGGCGGCGACAAAAGCAGC
CAACAGCGTGACATCAAGGCAGCCCACCAGATTCTGCGTTCGTGGAGGATGCAAAATGACTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 4.7906

>AT22638 WP_012273611.1 NC_010322:4488291-4488584 [Pseudomonas putida GB-1]
MTETFSEFDPAEYLTTPEAIAQFMTDALETGDAGYVAKAVGVVARAKGMSELAKETGLSREQLYRSFSEHGNPTLKSFLT
VMKALGIDMTARPHLAG

Download         Length: 294 bp

>AT22638 NC_010322:4488291-4488584 [Pseudomonas putida GB-1]
ATGACTGAAACCTTTTCCGAATTCGACCCCGCCGAGTACCTCACAACGCCCGAAGCAATCGCGCAGTTCATGACCGACGC
ACTTGAGACTGGCGATGCCGGCTATGTTGCCAAAGCGGTTGGCGTGGTGGCCCGTGCAAAGGGCATGAGCGAACTGGCCA
AAGAAACTGGCCTGTCTCGCGAACAGCTCTACCGCTCTTTCAGCGAGCACGGCAACCCGACCCTGAAGTCGTTCCTGACC
GTAATGAAGGCATTAGGCATAGACATGACGGCGCGCCCCCACCTTGCTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Y3XTI2

Antitoxin

Source ID Structure
AlphaFold DB A0A7Y3XSY8

References