Detailed information of TA system TA022636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 2331057..2331940
• Replicon: chromosome
• Accession: NC_010322
• Organism: Pseudomonas putida GB-1

Toxin (Protein)

• Gene name: -
• Uniprot ID: B0KLK3
• Locus tag: PPUTGB1_RS10640
• Protein ID: WP_012271731.1
• Coordinates: 2331503..2331940 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: B0KLK2
• Locus tag: PPUTGB1_RS10635
• Protein ID: WP_012271730.1
• Coordinates: 2331057..2331506 (+)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPUTGB1_RS10610	2326503..2327702	+	1200	WP_012271725.1	sugar transporter	-
PPUTGB1_RS10615	2327753..2328337	-	585	WP_012271726.1	LysE family transporter	-
PPUTGB1_RS10620	2328388..2329218	-	831	WP_080516246.1	AraC family transcriptional regulator	-
PPUTGB1_RS10625	2329267..2330190	-	924	WP_041166461.1	LysR family transcriptional regulator	-
PPUTGB1_RS10630	2330294..2330947	+	654	WP_012271729.1	oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB	-
PPUTGB1_RS10635	2331057..2331506	+	450	WP_012271730.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
PPUTGB1_RS10640	2331503..2331940	+	438	WP_012271731.1	RES family NAD+ phosphorylase	Toxin
PPUTGB1_RS10645	2331948..2333102	-	1155	WP_012271732.1	aminotransferase class V-fold PLP-dependent enzyme	-
PPUTGB1_RS10650	2333297..2334223	-	927	WP_012271733.1	LysR family transcriptional regulator	-
PPUTGB1_RS10655	2334364..2335593	+	1230	WP_012271734.1	acyl-CoA dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-138)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (33-91)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 16000.32 Da
• Isoelectric Point: 6.2187

>T22636 WP_012271731.1 NC_010322:2331503-2331940 [Pseudomonas putida GB-1]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPPGAMLEVLVHLEIDPEDFPTTLRLIRIELPDTVSQARLPTLQ
PGWSAQPELTRALGNRFLDDCSALLLPVPSAIMPSTTNYLFNPRHPQAQSAKIQVEDFTPDSRLF

Nucleotide

• Length: 438 bp

>T22636 NC_010322:2331503-2331940 [Pseudomonas putida GB-1]
GTGATTCTGTGGCGTATCAGCGCCTACGCAGACTTGAGCGGCACGGGAGGCTTGCGGGTAAGCGGCCGTTGGCACCAGGC
GGGCCGGCCGGTGGTATATGCCGCCACCAGCCCTCCAGGGGCGATGCTCGAGGTGTTGGTGCACCTGGAGATCGACCCTG
AGGATTTCCCCACTACCCTGCGCCTGATCCGCATCGAATTGCCCGACACGGTTTCACAAGCGCGGTTACCCACCTTGCAG
CCAGGCTGGTCCGCTCAGCCGGAGTTGACCCGGGCACTGGGCAATCGCTTTCTGGATGACTGTTCGGCATTGTTGTTGCC
GGTGCCGAGCGCGATCATGCCCAGTACCACCAACTACCTGTTCAACCCACGGCACCCGCAGGCGCAGAGCGCGAAAATAC
AGGTCGAGGATTTCACCCCGGACAGCCGCTTGTTCTAG

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 17034.57 Da
• Isoelectric Point: 6.1074

>AT22636 WP_012271730.1 NC_010322:2331057-2331506 [Pseudomonas putida GB-1]
MLAEVLRDNGYHEYRARLNALLEIPELASDFEIHTRITHGFAATWLVKLTERGVLTPVERDQIIPLRTLKTRIERDQPLT
VDESDRLFRSAHITAMAEAVFGEADKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGFGL

Nucleotide

• Length: 450 bp

>AT22636 NC_010322:2331057-2331506 [Pseudomonas putida GB-1]
ATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCATGAGTACCGGGCGCGTTTGAACGCGCTGCTGGAAATCCCCGAGCT
TGCCAGTGACTTTGAAATCCACACCCGCATCACCCATGGTTTTGCCGCCACCTGGCTGGTAAAGCTGACCGAGCGTGGTG
TGCTCACGCCGGTGGAGCGCGACCAGATCATTCCCCTGCGCACCCTGAAAACCCGCATTGAGCGCGACCAGCCGTTGACC
GTGGATGAAAGCGACCGTCTGTTCCGCTCGGCCCACATCACCGCCATGGCCGAAGCCGTGTTCGGCGAGGCGGACAAGGC
CAAACGTTGGTTGTCCAAGCCCAAGGAGCGCTTTTCGGGGCTCACCCCGATGCAGATGCTTACCACCCAGCAAGGCACCA
CTCAGGTTGAAGAGATGCTGCTGCAGATTGCCGAGGGCTTTGGCCTGTGA

Structures

Toxin

• AlphaFold DB: B0KLK3

Antitoxin

• AlphaFold DB: A0A7Y3UUY4