Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 986482..987110 Replicon chromosome
Accession NC_010320
Organism Thermoanaerobacter sp. X514

Toxin (Protein)


Gene name mazF Uniprot ID B0KD57
Locus tag TETH514_RS04940 Protein ID WP_003867472.1
Coordinates 986760..987110 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID B0KD56
Locus tag TETH514_RS04935 Protein ID WP_003867471.1
Coordinates 986482..986757 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TETH514_RS04910 981810..982175 + 366 WP_003867466.1 hypothetical protein -
TETH514_RS04915 982271..982687 + 417 WP_003867467.1 holo-ACP synthase -
TETH514_RS04920 982650..984182 + 1533 WP_009052807.1 bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase -
TETH514_RS04925 984194..985204 + 1011 WP_003867469.1 outer membrane lipoprotein carrier protein LolA -
TETH514_RS04930 985226..986392 + 1167 WP_003867470.1 alanine racemase -
TETH514_RS04935 986482..986757 + 276 WP_003867471.1 ribbon-helix-helix protein, CopG family Antitoxin
TETH514_RS04940 986760..987110 + 351 WP_003867472.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
TETH514_RS04945 987438..988283 + 846 WP_003867473.1 YitT family protein -
TETH514_RS04950 988299..989120 + 822 WP_012268649.1 transketolase -
TETH514_RS04955 989121..990041 + 921 WP_003870015.1 transketolase family protein -
TETH514_RS04960 990070..990585 - 516 WP_003871225.1 hypothetical protein -
TETH514_RS04965 990769..992013 + 1245 WP_009052805.1 6-phosphofructokinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12933.02 Da        Isoelectric Point: 6.4714

>T22634 WP_003867472.1 NC_010320:986760-987110 [Thermoanaerobacter sp. X514]
MVIKRGDIFYADLSPVIGSEQGGIRPVLIIQNDIGNKYSPTVIVAAITSQINKAKLPTHVEINGAEYGLNKDSVVLLEQI
RTIDKKRLREKIGHFDQEMMEKVNEALQISLGLIDF

Download         Length: 351 bp

>T22634 NC_010320:986760-987110 [Thermoanaerobacter sp. X514]
ATGGTGATAAAGAGGGGAGACATTTTTTATGCCGATTTAAGCCCTGTAATAGGCTCTGAACAGGGCGGAATTCGGCCTGT
ACTTATAATTCAAAATGATATTGGTAATAAATATAGTCCAACAGTAATAGTAGCGGCAATTACATCTCAGATTAACAAAG
CCAAATTGCCCACTCATGTTGAAATAAATGGGGCAGAATATGGACTTAACAAAGATTCTGTAGTATTATTGGAGCAAATA
AGGACTATTGACAAAAAACGTTTAAGAGAAAAAATTGGTCATTTTGACCAGGAAATGATGGAGAAAGTCAATGAAGCTTT
GCAAATAAGTTTAGGGTTGATTGATTTTTAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10533.33 Da        Isoelectric Point: 6.3661

>AT22634 WP_003867471.1 NC_010320:986482-986757 [Thermoanaerobacter sp. X514]
VGETKRILVSLPQSLLEEVDVLAAMENRNRSEFIREAMKLYIREKKKIQIRESMKKGYLEMAAINSELAEMGLTAENECF
TGYEMKLKKCD

Download         Length: 276 bp

>AT22634 NC_010320:986482-986757 [Thermoanaerobacter sp. X514]
GTGGGCGAAACAAAGCGAATACTTGTAAGCTTGCCGCAAAGTTTATTAGAAGAAGTTGATGTTCTTGCCGCTATGGAAAA
CAGGAATCGCAGTGAATTTATAAGAGAAGCGATGAAATTATATATACGCGAGAAGAAAAAAATTCAAATACGAGAGAGCA
TGAAAAAGGGATATCTTGAGATGGCAGCAATTAACAGTGAACTTGCGGAAATGGGCCTAACCGCAGAAAACGAATGTTTT
ACAGGATATGAAATGAAATTGAAAAAGTGTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B3BRL3

Antitoxin

Source ID Structure
AlphaFold DB A0A1M4W2H3

References