Detailed information of TA system TA022630

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 2793968..2794827
• Replicon: chromosome
• Accession: NC_010296
• Organism: Microcystis aeruginosa NIES-843

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: B0JKU9
• Locus tag: MAE_RS13360
• Protein ID: WP_012266042.1
• Coordinates: 2794429..2794827 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: B0JKU8
• Locus tag: MAE_RS13355
• Protein ID: WP_012266041.1
• Coordinates: 2793968..2794387 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAE_RS13340	2789432..2790601	+	1170	WP_012266038.1	trypsin-like peptidase domain-containing protein	-
MAE_RS13345	2790670..2791533	-	864	WP_012266039.1	WYL domain-containing protein	-
MAE_RS13350	2791633..2793798	+	2166	WP_012266040.1	type I-D CRISPR-associated helicase Cas3'	-
MAE_RS13355	2793968..2794387	+	420	WP_012266041.1	nucleotidyltransferase domain-containing protein	Antitoxin
MAE_RS13360	2794429..2794827	+	399	WP_012266042.1	DUF86 domain-containing protein	Toxin
MAE_RS13365	2794820..2795278	+	459	WP_012266043.1	nucleotidyltransferase domain-containing protein	-
MAE_RS13370	2795282..2795716	+	435	WP_002778659.1	DUF86 domain-containing protein	-
MAE_RS13380	2795709..2799217	+	3509	Protein_2749	type I-D CRISPR-associated protein Cas10d/Csc3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (1-117)

Antitoxin

• Polymerase beta, nucleotidyltransferase (6-101)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15386.66 Da
• Isoelectric Point: 4.6345

>T22630 WP_012266042.1 NC_010296:2794429-2794827 [Microcystis aeruginosa NIES-843]
MNQYLAELSEYGSITLEDYRTLRERQLAIERLIQLIVQTGIDINYQILKCLDIESPNNARDALFQIVELGILEEHLAVQL
AESIKLRNLLVHLYKKIDPDIVHSSIANILRDYPRYQRSIVQYLDFLEAENG

Nucleotide

• Length: 399 bp

>T22630 NC_010296:2794429-2794827 [Microcystis aeruginosa NIES-843]
ATGAATCAATACCTCGCCGAACTTAGCGAGTACGGTTCGATCACCTTAGAGGATTATCGTACATTAAGAGAACGTCAATT
AGCCATAGAAAGGTTAATTCAGCTTATCGTTCAAACTGGCATCGATATCAATTACCAGATTTTAAAATGTTTAGATATCG
AGTCTCCGAATAATGCTCGCGACGCTTTATTTCAAATCGTAGAACTGGGAATTTTAGAAGAACACCTAGCCGTTCAACTG
GCCGAGTCTATTAAATTAAGAAACTTACTCGTACACCTATACAAGAAAATTGATCCCGATATCGTTCATTCCTCGATCGC
TAATATTTTACGAGATTATCCCCGCTACCAACGCTCGATCGTTCAATATCTAGATTTCTTGGAGGCAGAAAATGGTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 16152.58 Da
• Isoelectric Point: 4.9146

>AT22630 WP_012266041.1 NC_010296:2793968-2794387 [Microcystis aeruginosa NIES-843]
MNRLVEKITAIVEKLPNLKLLILFGSRARGEHKPDSDWDFAVLYEERSDQKDISSLLKIYSLLEQALEIPDDKIDVVDLK
ECSPILAHYVARDGQLLYERETGIFEGFKEKFLMNPEESKALYRQLRNNLEISLREKGV

Nucleotide

• Length: 420 bp

>AT22630 NC_010296:2793968-2794387 [Microcystis aeruginosa NIES-843]
ATGAATAGGCTAGTAGAAAAAATTACGGCTATTGTCGAAAAACTACCGAATTTAAAACTATTAATTCTCTTCGGTTCTAG
AGCGCGTGGAGAGCATAAACCTGATAGCGATTGGGATTTCGCTGTTTTATACGAGGAAAGAAGCGATCAAAAGGATATTT
CGAGTCTATTGAAAATTTATTCACTGCTCGAACAAGCTTTAGAGATTCCCGATGACAAAATTGATGTGGTTGATTTAAAA
GAATGTTCGCCGATCCTCGCTCATTATGTCGCACGGGATGGTCAACTTCTATACGAACGGGAAACGGGAATATTCGAGGG
ATTTAAAGAAAAATTTCTCATGAATCCTGAAGAATCGAAAGCGTTATACCGTCAATTACGGAATAATCTAGAGATTTCTC
TACGAGAAAAAGGAGTGTAA

Structures

Toxin

• AlphaFold DB: B0JKU9

Antitoxin

• AlphaFold DB: B0JKU8