Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/DUF433(antitoxin)
Location 2765194..2765765 Replicon chromosome
Accession NC_010296
Organism Microcystis aeruginosa NIES-843

Toxin (Protein)


Gene name vapC Uniprot ID A0A3G9JC50
Locus tag MAE_RS13240 Protein ID WP_041804105.1
Coordinates 2765194..2765535 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID I4I6X1
Locus tag MAE_RS13245 Protein ID WP_002799619.1
Coordinates 2765532..2765765 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAE_RS13225 2760736..2761779 + 1044 WP_012266014.1 IS630 family transposase -
MAE_RS13230 2761895..2763631 - 1737 WP_012266015.1 IS1634 family transposase -
MAE_RS33370 2763699..2763854 - 156 WP_012266016.1 hypothetical protein -
MAE_RS13235 2764280..2765122 - 843 WP_012266017.1 IS5 family transposase -
MAE_RS13240 2765194..2765535 - 342 WP_041804105.1 DUF5615 family PIN-like protein Toxin
MAE_RS13245 2765532..2765765 - 234 WP_002799619.1 DUF433 domain-containing protein Antitoxin
MAE_RS34095 2766014..2766154 + 141 WP_164516965.1 hypothetical protein -
MAE_RS13250 2766416..2766712 - 297 WP_012266020.1 hypothetical protein -
MAE_RS13255 2767275..2768759 - 1485 WP_012264152.1 IS1380-like element ISMae9 family transposase -
MAE_RS13260 2769170..2769478 - 309 WP_041804106.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2760736..2761779 1043
- flank IS/Tn - - 2767275..2768759 1484


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Domain of unknown function DUF5615

(1-78)

Antitoxin

Protein of unknown function DUF433

(11-66)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12506.41 Da        Isoelectric Point: 4.3569

>T22629 WP_041804105.1 NC_010296:c2765535-2765194 [Microcystis aeruginosa NIES-843]
MKIWIDAQLPPTLANWLSATFGLEASALRDLSLRDAQDINIFEAARAENAVIMTKDSDFIDLVCRLGTPPQILWLTCGNV
TNRNLRQLLSATLPDALEQLRQGTMIVEISNAP

Download         Length: 342 bp

>T22629 NC_010296:c2765535-2765194 [Microcystis aeruginosa NIES-843]
ATGAAAATTTGGATTGATGCCCAACTACCTCCAACGCTGGCGAACTGGTTATCAGCGACTTTTGGTTTAGAAGCGTCTGC
TCTACGAGACTTGTCCCTTCGAGATGCTCAAGACATCAATATCTTTGAAGCAGCGCGAGCCGAAAATGCCGTGATTATGA
CTAAAGATAGCGACTTCATTGATTTGGTCTGTCGCCTGGGAACACCTCCCCAAATTTTATGGTTGACCTGTGGTAATGTC
ACGAATCGGAATCTGCGGCAATTGCTGTCAGCTACTTTACCTGATGCTTTAGAACAACTACGTCAAGGAACAATGATCGT
AGAGATCAGCAATGCTCCTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8592.99 Da        Isoelectric Point: 4.6320

>AT22629 WP_002799619.1 NC_010296:c2765765-2765532 [Microcystis aeruginosa NIES-843]
MNSTVSLLTRITQTPGQCGGRPCIRGMRIRVTDILEMLAENVSINEILEDFPDLELADIQACLLFAARRTDFPRLTA

Download         Length: 234 bp

>AT22629 NC_010296:c2765765-2765532 [Microcystis aeruginosa NIES-843]
ATGAACTCAACAGTTAGCTTGCTTACCCGCATTACTCAAACACCTGGTCAGTGTGGTGGTCGTCCTTGCATCCGAGGGAT
GCGAATTCGAGTGACTGATATTTTAGAAATGTTGGCTGAGAACGTTAGCATTAATGAAATTTTAGAAGACTTCCCCGATT
TGGAACTAGCAGATATCCAAGCTTGTTTGCTTTTTGCAGCTCGCCGTACTGATTTCCCCAGACTAACGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9JC50

Antitoxin

Source ID Structure
AlphaFold DB I4I6X1

References