Detailed information of TA system TA022624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 334512..335008
• Replicon: chromosome
• Accession: NC_010296
• Organism: Microcystis aeruginosa NIES-843

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: I4IHG7
• Locus tag: MAE_RS01725
• Protein ID: WP_002768607.1
• Coordinates: 334820..335008 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: I4IHG6
• Locus tag: MAE_RS01720
• Protein ID: WP_002763050.1
• Coordinates: 334512..334808 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAE_RS33080	329772..329945	+	174	WP_155120266.1	hypothetical protein	-
MAE_RS01680	330213..330449	+	237	WP_041803658.1	DUF4160 domain-containing protein	-
MAE_RS01685	330433..330681	+	249	WP_002785172.1	DUF2442 domain-containing protein	-
MAE_RS01690	331150..332241	-	1092	WP_012264046.1	ISAs1-like element ISMae8 family transposase	-
MAE_RS01695	332417..332758	-	342	WP_012264047.1	DUF86 domain-containing protein	-
MAE_RS01700	332755..333048	-	294	WP_002763046.1	nucleotidyltransferase family protein	-
MAE_RS01705	333135..333314	-	180	Protein_343	hypothetical protein	-
MAE_RS01710	333327..333671	-	345	WP_002798281.1	hypothetical protein	-
MAE_RS01715	333680..334027	-	348	WP_012264049.1	hypothetical protein	-
MAE_RS01720	334512..334808	-	297	WP_002763050.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MAE_RS01725	334820..335008	-	189	WP_002768607.1	type II toxin-antitoxin system HicA family toxin	Toxin
MAE_RS27605	335428..339567	-	4140	WP_012264052.1	serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	331150..332271	1121

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-91)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7161.44 Da
• Isoelectric Point: 10.8174

>T22624 WP_002768607.1 NC_010296:c335008-334820 [Microcystis aeruginosa NIES-843]
MESREIIKKLKDEGWYQVGTTGSHHHFKHPHKKGKVTVPHPKKDIPLKTLISIEKQAGIKLR

Nucleotide

• Length: 189 bp

>T22624 NC_010296:c335008-334820 [Microcystis aeruginosa NIES-843]
ATGGAAAGTCGAGAAATTATCAAAAAACTGAAGGATGAGGGATGGTATCAAGTAGGTACAACAGGAAGTCATCATCATTT
TAAACATCCCCATAAAAAAGGGAAAGTGACTGTTCCTCACCCTAAAAAGGATATTCCCCTTAAAACCCTAATCAGTATCG
AAAAACAAGCCGGTATTAAATTACGATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10797.16 Da
• Isoelectric Point: 4.1825

>AT22624 WP_002763050.1 NC_010296:c334808-334512 [Microcystis aeruginosa NIES-843]
MITYLATVHKDNHSDYGVQFYDFPGCISAGETIEEAKKMATEALKGHISFMLADGDEIPTPSTLETILTDADHQDAIAFL
LIEVSEAILNHQELCVKN

Nucleotide

• Length: 297 bp

>AT22624 NC_010296:c334808-334512 [Microcystis aeruginosa NIES-843]
ATGATTACTTATTTAGCCACCGTTCATAAAGATAACCATAGTGATTATGGGGTACAATTTTATGACTTTCCGGGTTGTAT
TAGTGCAGGAGAAACCATCGAAGAAGCGAAAAAAATGGCAACTGAAGCCTTAAAAGGTCATATTTCCTTTATGTTGGCAG
ATGGGGACGAAATTCCCACCCCTAGCACCCTCGAAACCATTTTAACCGACGCGGATCATCAAGATGCGATCGCTTTTCTG
CTCATTGAGGTATCAGAAGCTATTTTAAACCATCAGGAATTGTGTGTAAAAAACTAG

Structures

Toxin

• AlphaFold DB: I4IHG7

Antitoxin

• AlphaFold DB: I4IHG6