Detailed information of TA system TA022623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2013440..2014155
• Replicon: chromosome
• Accession: NC_010278
• Organism: Actinobacillus pleuropneumoniae serovar 3 str. JL03

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: APJL_RS09425
• Protein ID: WP_012263404.1
• Coordinates: 2013440..2013868 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: APJL_RS09430
• Protein ID: WP_005599447.1
• Coordinates: 2013868..2014155 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APJL_RS09410 (APJL_1861)	2009025..2010731	-	1707	WP_005606484.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
APJL_RS09415 (APJL_1862)	2010827..2011108	-	282	WP_012263402.1	DNA-directed RNA polymerase subunit omega	-
APJL_RS09420 (APJL_1863)	2011235..2013316	-	2082	WP_012263403.1	ATP-dependent DNA helicase RecG	-
APJL_RS09425 (APJL_1864)	2013440..2013868	-	429	WP_012263404.1	type II toxin-antitoxin system VapC family toxin	Toxin
APJL_RS09430 (APJL_1865)	2013868..2014155	-	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
APJL_RS09435 (APJL_1867)	2014361..2016076	+	1716	WP_012263406.1	proline--tRNA ligase	-
APJL_RS09440 (APJL_1868)	2016142..2016330	-	189	WP_005602684.1	hypothetical protein	-
APJL_RS09445 (APJL_1869)	2016333..2016914	-	582	WP_012263407.1	sigma-70 family RNA polymerase sigma factor	-
APJL_RS09450 (APJL_1870)	2017003..2017959	+	957	WP_012263408.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
APJL_RS09455 (APJL_1871)	2017959..2018552	+	594	WP_005599453.1	sulfoxide reductase heme-binding subunit YedZ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 16495.92 Da
• Isoelectric Point: 7.7945

>T22623 WP_012263404.1 NC_010278:c2013868-2013440 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MFQYLFDTNIISELYKLGNSRIDTNVRQWLETIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQA
KSFSINNEIALLASEYHIPNKMDLNDAYIAATAKYHNLVLVTRNLKDFNCCDIRLFNPFEPN

Nucleotide

• Length: 429 bp

>T22623 NC_010278:c2013868-2013440 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGTTTCAATATCTTTTTGATACCAACATCATTTCCGAGCTTTATAAGCTAGGAAACAGTAGAATAGATACCAATGTACG
ACAATGGTTAGAAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAATAAAAACGGGGATTTTAC
TTAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTATTAAGCGTTTATCAAGCT
AAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAAATGGATTTAAACGATGC
TTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTTCAACTGTTGCGATATAC
GTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT22623 WP_005599447.1 NC_010278:c2014155-2013868 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT22623 NC_010278:c2014155-2013868 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5