Detailed information of TA system TA022621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1713587..1714318
• Replicon: chromosome
• Accession: NC_010278
• Organism: Actinobacillus pleuropneumoniae serovar 3 str. JL03

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: B0BRD2
• Locus tag: APJL_RS07850
• Protein ID: WP_005605461.1
• Coordinates: 1713587..1714054 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: APJL_RS07855
• Protein ID: WP_005619269.1
• Coordinates: 1714058..1714318 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APJL_RS07825 (APJL_1560)	1708942..1709313	-	372	WP_005598830.1	CrcB family protein	-
APJL_RS07830 (APJL_1561)	1709316..1710380	-	1065	WP_011848610.1	MFS transporter	-
APJL_RS07835 (APJL_1562)	1710475..1711584	+	1110	WP_012263272.1	anhydro-N-acetylmuramic acid kinase	-
APJL_RS07840 (APJL_1563)	1711638..1712552	+	915	WP_012263273.1	N-acetylmuramic acid 6-phosphate etherase	-
APJL_RS07845 (APJL_1564)	1712622..1713503	-	882	WP_012263274.1	50S ribosomal protein L11 methyltransferase	-
APJL_RS07850 (APJL_1565)	1713587..1714054	-	468	WP_005605461.1	GNAT family N-acetyltransferase	Toxin
APJL_RS07855 (APJL_1566)	1714058..1714318	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
APJL_RS07860 (APJL_1567)	1714439..1715899	-	1461	WP_012263275.1	metalloprotease TldD	-
APJL_RS07865 (APJL_1568)	1716029..1717288	+	1260	WP_005618010.1	tRNA lysidine(34) synthetase TilS	-
APJL_RS07870 (APJL_1569)	1717315..1717605	-	291	WP_005602215.1	DUF5389 family protein	-
APJL_RS07875 (APJL_1570)	1717607..1718500	-	894	WP_005608892.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (40-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17276.25 Da
• Isoelectric Point: 9.5125

>T22621 WP_005605461.1 NC_010278:c1714054-1713587 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVRDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGVKALLVHALNQQAKQFYLKYGFSCSPIDEMVLMLKL

Nucleotide

• Length: 468 bp

>T22621 NC_010278:c1714054-1713587 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGCGTGATGAGAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGCCATCAGTTTGTTGCAGGCGCATTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGTTCGGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTAGCGAATCAAATCGGGGTAAAAGCATTGCTTGTTCATGCGTTAAATCAGCAAGCAAAAC
AATTTTATTTAAAGTACGGTTTTTCATGTTCACCCATTGATGAGATGGTTTTAATGTTAAAACTTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT22621 WP_005619269.1 NC_010278:c1714318-1714058 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT22621 NC_010278:c1714318-1714058 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGCATTTTAGGTAA
AACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACCGTTTTCCAATTAA
ATGAGCAGGCTTTCGAGCAATTTAACCGATTGCTAGAACAGCCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Toxin

• AlphaFold DB: B0BRD2

Antitoxin

• AlphaFold DB: A3N2I7