Detailed information of TA system TA022620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 1511568..1512213
• Replicon: chromosome
• Accession: NC_010278
• Organism: Actinobacillus pleuropneumoniae serovar 3 str. JL03

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: B0BQU7
• Locus tag: APJL_RS06855
• Protein ID: WP_005619674.1
• Coordinates: 1511842..1512213 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: APJL_RS06850
• Protein ID: WP_005598477.1
• Coordinates: 1511568..1511861 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APJL_RS06820 (APJL_1369)	1506900..1507583	-	684	WP_012263193.1	arginine ABC transporter permease ArtM	-
APJL_RS06825 (APJL_1370)	1507583..1508254	-	672	WP_005612835.1	arginine ABC transporter permease ArtQ	-
APJL_RS06830 (APJL_1371)	1508260..1508994	-	735	WP_009874916.1	transporter substrate-binding domain-containing protein	-
APJL_RS06835 (APJL_1372)	1508999..1509733	-	735	WP_005598473.1	arginine ABC transporter ATP-binding protein ArtP	-
APJL_RS06840 (APJL_1373)	1509958..1510455	-	498	WP_005601953.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
APJL_RS06845 (APJL_1374)	1510528..1511490	+	963	WP_012263194.1	calcium/sodium antiporter	-
APJL_RS06850 (APJL_1375)	1511568..1511861	-	294	WP_005598477.1	helix-turn-helix domain-containing protein	Antitoxin
APJL_RS06855 (APJL_1376)	1511842..1512213	-	372	WP_005619674.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
APJL_RS06860 (APJL_1377)	1512407..1514158	+	1752	WP_043948002.1	protein-disulfide reductase DsbD	-
APJL_RS06865 (APJL_1378)	1514216..1514785	+	570	WP_005605204.1	elongation factor P hydroxylase	-
APJL_RS06870 (APJL_1379)	1514829..1515683	-	855	WP_005598486.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (32-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-85)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 15066.57 Da
• Isoelectric Point: 10.4054

>T22620 WP_005619674.1 NC_010278:c1512213-1511842 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MYEIVFYRDKRGREPVKEFLLRLLKERQEGSRERLHKISHHLSILHLHGTRAGENYIKHLEDRIWQLRPVGDCLLFASII
RGKFVLLHYFAKQNYRIPKREIERAKVRLADLQERIKDEPYWF

Nucleotide

• Length: 372 bp

>T22620 NC_010278:c1512213-1511842 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGTATGAAATAGTCTTTTATCGAGATAAGCGGGGGAGAGAACCCGTAAAAGAATTTCTGTTAAGGCTACTAAAAGAACG
GCAGGAAGGCAGTCGAGAACGATTACACAAAATCAGCCATCATTTGAGTATTTTACATTTGCACGGTACTCGAGCCGGTG
AGAATTATATTAAGCATTTGGAAGATCGTATTTGGCAGTTACGTCCGGTGGGCGATTGCCTGTTATTTGCAAGTATTATT
CGGGGAAAATTTGTGTTGTTGCATTATTTTGCCAAACAGAATTATCGAATTCCGAAACGAGAAATAGAACGTGCAAAAGT
TCGGTTGGCGGATTTACAGGAGAGAATAAAAGATGAGCCCTATTGGTTCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10935.81 Da
• Isoelectric Point: 10.3120

>AT22620 WP_005598477.1 NC_010278:c1511861-1511568 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MSPIGSSWTSFEQEIFDESEIAETQFRIRLILEIIETRQQLGISQRKLEKLSGVKQSMIARVEKGSVNPSLATVLKLLVP
LGKTLQIVPLDKNKTVR

Nucleotide

• Length: 294 bp

>AT22620 NC_010278:c1511861-1511568 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGAGCCCTATTGGTTCTAGTTGGACATCGTTTGAACAAGAAATTTTTGACGAGTCGGAAATAGCGGAAACTCAGTTTCG
CATACGACTTATTTTAGAAATCATTGAAACCCGTCAGCAGCTCGGTATTTCTCAACGAAAATTGGAGAAATTAAGCGGTG
TAAAACAATCTATGATTGCTCGGGTAGAAAAAGGCTCGGTTAATCCGTCGTTGGCGACGGTATTAAAGTTGCTTGTGCCG
TTGGGGAAAACTTTACAAATCGTACCGTTAGATAAAAACAAAACGGTACGTTAA

Structures

Toxin

• AlphaFold DB: A3N206