Detailed information of TA system TA022619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 1417602..1418110
• Replicon: chromosome
• Accession: NC_010278
• Organism: Actinobacillus pleuropneumoniae serovar 3 str. JL03

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B0BQK9
• Locus tag: APJL_RS06380
• Protein ID: WP_005605064.1
• Coordinates: 1417850..1418110 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B0BQK8
• Locus tag: APJL_RS06375
• Protein ID: WP_005598318.1
• Coordinates: 1417602..1417853 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APJL_RS06335 (APJL_1284)	1413137..1413685	-	549	WP_012263163.1	type IV pilus biogenesis/stability protein PilW	-
APJL_RS06340 (APJL_1285)	1413739..1414920	-	1182	WP_005608495.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
APJL_RS06365 (APJL_1286)	1415825..1417264	+	1440	WP_005605060.1	glutamate--tRNA ligase	-
APJL_RS06375 (APJL_1287)	1417602..1417853	+	252	WP_005598318.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
APJL_RS06380 (APJL_1288)	1417850..1418110	+	261	WP_005605064.1	Txe/YoeB family addiction module toxin	Toxin
APJL_RS06385 (APJL_1289)	1418268..1418435	-	168	WP_009875287.1	Trm112 family protein	-
APJL_RS06390 (APJL_1290)	1418437..1419417	-	981	WP_005608502.1	tetraacyldisaccharide 4'-kinase	-
APJL_RS06395 (APJL_1291)	1419511..1420770	-	1260	WP_005598324.1	ATP-dependent protease ATP-binding subunit ClpX	-
APJL_RS06400 (APJL_1292)	1420770..1421360	-	591	WP_005598326.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
APJL_RS06405 (APJL_1293)	1421484..1421876	+	393	WP_012263165.1	SufE family protein	-
APJL_RS06420 (APJL_1294)	1422235..1423008	-	774	WP_012263166.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10423.99 Da
• Isoelectric Point: 8.0109

>T22619 WP_005605064.1 NC_010278:1417850-1418110 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKLEPLKFNLSGFWSRRINEEHRLIYSVEDEAILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T22619 NC_010278:1417850-1418110 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9362.66 Da
• Isoelectric Point: 4.5659

>AT22619 WP_005598318.1 NC_010278:1417602-1417853 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYCSLEETAYLMRSPTNAKRLLDSVAELRQGQGIERE
LIE

Nucleotide

• Length: 252 bp

>AT22619 NC_010278:1417602-1417853 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACGATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TGATGCGTTCACCCACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Toxin

• AlphaFold DB: B0BQK9

Antitoxin

• AlphaFold DB: B0BQK8