Detailed information of TA system TA022618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 722007..722657
• Replicon: chromosome
• Accession: NC_010278
• Organism: Actinobacillus pleuropneumoniae serovar 3 str. JL03

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B0BNU3
• Locus tag: APJL_RS03225
• Protein ID: WP_005614926.1
• Coordinates: 722007..722396 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BNU4
• Locus tag: APJL_RS03230
• Protein ID: WP_005604117.1
• Coordinates: 722397..722657 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APJL_RS03215 (APJL_0657)	717247..720522	-	3276	WP_012262906.1	DEAD/DEAH box helicase family protein	-
APJL_RS03220 (APJL_0658)	720648..721871	-	1224	WP_012262907.1	ATP-binding protein	-
APJL_RS03225 (APJL_0659)	722007..722396	-	390	WP_005614926.1	type II toxin-antitoxin system VapC family toxin	Toxin
APJL_RS03230 (APJL_0660)	722397..722657	-	261	WP_005604117.1	hypothetical protein	Antitoxin
APJL_RS03235 (APJL_0661)	723003..723839	+	837	WP_012262908.1	hypothetical protein	-
APJL_RS03240 (APJL_0662)	723871..725892	-	2022	WP_012262909.1	excinuclease ABC subunit B	-
APJL_RS03245 (APJL_0663)	726069..726881	+	813	WP_005607533.1	EfeM/EfeO family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14837.17 Da
• Isoelectric Point: 5.1295

>T22618 WP_005614926.1 NC_010278:c722396-722007 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MYMLDTNTVSYFFRQDPTVVKKLQQLNPELICISSVTAAELFYGVKKRNNQKLTAFLNTFLSAITVMDWDYQVAEVYGQL
RAEMEREGKIMGVQDQMIGAHALATECVLVSSDKAFQFIPNLVLENWWK

Nucleotide

• Length: 390 bp

>T22618 NC_010278:c722396-722007 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGTATATGCTAGATACTAATACGGTGAGTTATTTCTTTAGGCAAGATCCTACTGTGGTTAAGAAACTACAACAACTTAA
TCCGGAATTGATTTGTATTTCATCGGTCACGGCTGCAGAGTTGTTTTATGGCGTAAAAAAACGTAATAATCAGAAACTTA
CTGCATTTTTAAATACTTTTTTATCTGCGATTACGGTGATGGATTGGGATTATCAAGTGGCGGAAGTTTATGGTCAGCTG
CGAGCAGAGATGGAACGAGAAGGCAAAATCATGGGCGTTCAAGATCAAATGATTGGAGCTCATGCCCTAGCAACAGAATG
TGTTCTTGTTTCCAGCGATAAAGCCTTTCAATTTATCCCTAATCTCGTATTAGAAAATTGGTGGAAGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10289.63 Da
• Isoelectric Point: 5.1867

>AT22618 WP_005604117.1 NC_010278:c722657-722397 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MEKVARLFRNGRNQAVRLPVEFEFNAKQVYVFKEANGDITLSTRSRNQQNWQKVFELLPMLECDEQFLSEQERKQEMATR
DPFEGF

Nucleotide

• Length: 261 bp

>AT22618 NC_010278:c722657-722397 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGGAAAAGGTCGCACGTTTATTTAGAAACGGACGTAATCAAGCCGTACGATTACCGGTTGAATTTGAGTTTAATGCGAA
GCAAGTTTATGTGTTTAAAGAAGCAAACGGCGATATTACGCTTTCGACACGTTCTCGCAATCAGCAAAATTGGCAGAAAG
TCTTTGAGTTATTACCAATGCTTGAATGTGATGAACAGTTTTTATCGGAACAAGAACGCAAGCAAGAAATGGCAACTCGA
GATCCGTTCGAGGGCTTTTAA

Structures

Toxin

• AlphaFold DB: A3N029

Antitoxin

• AlphaFold DB: A3N030