Detailed information of TA system TA226174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): AbkB-shpB/BrnT-BrnA
• Location: 1697918..1698470
• Replicon: chromosome
• Accession: NZ_CP087796
• Organism: Moraxella bovis strain SAM57957

Toxin (Protein)

• Gene name: AbkB
• Uniprot ID: A0A1T0A7D3
• Locus tag: LP101_RS08380
• Protein ID: WP_029102268.1
• Coordinates: 1697918..1698196 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: LP101_RS08385
• Protein ID: WP_078273473.1
• Coordinates: 1698183..1698470 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LP101_RS08350 (LP101_08290)	1693904..1694680	-	777	WP_264678118.1	nucleotidyltransferase domain-containing protein	-
LP101_RS08355 (LP101_08295)	1694793..1695143	+	351	WP_078273468.1	TIGR01244 family sulfur transferase	-
LP101_RS08360 (LP101_08300)	1695291..1695806	+	516	WP_264678117.1	hypothetical protein	-
LP101_RS08365 (LP101_08305)	1695907..1696089	+	183	WP_264683628.1	hypothetical protein	-
LP101_RS08370 (LP101_08310)	1696302..1696967	+	666	WP_264678115.1	PspA/IM30 family protein	-
LP101_RS08375 (LP101_08315)	1697167..1697823	+	657	WP_264678114.1	YqiJ family protein	-
LP101_RS08380 (LP101_08320)	1697918..1698196	+	279	WP_029102268.1	BrnT family toxin	Toxin
LP101_RS08385 (LP101_08325)	1698183..1698470	+	288	WP_078273473.1	BrnA antitoxin family protein	Antitoxin
LP101_RS08390 (LP101_08330)	1698480..1700210	+	1731	WP_078273474.1	flotillin domain-containing protein	-
LP101_RS08395	1700254..1700379	-	126	WP_264676709.1	hypothetical protein	-
LP101_RS08400 (LP101_08335)	1700408..1701928	-	1521	WP_264678113.1	DUF389 domain-containing protein	-
LP101_RS08405 (LP101_08340)	1702007..1703056	-	1050	WP_264678112.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-85)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (36-89)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10484.81 Da
• Isoelectric Point: 5.1084

>T226174 WP_029102268.1 NZ_CP087796:1697918-1698196 [Moraxella bovis]
MIFEWDDNKNRTNKQKHGVSFESASRIFLDPFLIVTEDTSSDEVRWLGVGSVDGVVILLVVHTHTDTDGTEIIRIISARK
LSRGEIERYGYC

Nucleotide

• Length: 279 bp

>T226174 NZ_CP122317:608304-608678 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10979.71 Da
• Isoelectric Point: 7.0097

>AT226174 WP_078273473.1 NZ_CP087796:1698183-1698470 [Moraxella bovis]
MAIVRKTLDDIKHTPIDKDELAQLQSMSDDSINYDDIPPLTDVQIANLRLYKPIKQQITLRLDSDIVAWLKKDGKGYQTR
ANQLLRQLMLKELTT

Nucleotide

• Length: 288 bp

>AT226174 NZ_CP122317:607847-608215 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A1T0A7D3