22616

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 242715..243357 Replicon chromosome
Accession NC_010184
Organism Bacillus weihenstephanensis KBAB4

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag BCERKBAB4_RS01340 Protein ID WP_000635965.1
Coordinates 243007..243357 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BCERKBAB4_RS01335 Protein ID WP_000004570.1
Coordinates 242715..243002 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCERKBAB4_RS01310 237880..238842 + 963 WP_012260209.1 UV DNA damage repair endonuclease UvsE -
BCERKBAB4_RS29250 239010..239558 - 549 WP_002139859.1 rhomboid family intramembrane serine protease -
BCERKBAB4_RS01320 239650..240009 + 360 WP_002009969.1 holo-ACP synthase -
BCERKBAB4_RS01325 240166..241116 + 951 WP_002124820.1 outer membrane lipoprotein carrier protein LolA -
BCERKBAB4_RS01330 241234..242403 + 1170 WP_002009971.1 alanine racemase -
BCERKBAB4_RS01335 242715..243002 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BCERKBAB4_RS01340 243007..243357 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BCERKBAB4_RS01345 243426..245594 + 2169 WP_002029442.1 RNA-binding transcriptional accessory protein -
BCERKBAB4_RS30780 245652..245768 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BCERKBAB4_RS01350 245979..246434 + 456 WP_016095131.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T22616 WP_000635965.1 NC_010184:243007-243357 [Bacillus mycoides KBAB4]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T22616 NC_010184:243007-243357 [Bacillus mycoides KBAB4]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT22616 WP_000004570.1 NC_010184:242715-243002 [Bacillus mycoides KBAB4]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT22616 NC_010184:242715-243002 [Bacillus mycoides KBAB4]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References