Detailed information of TA system TA022611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 4344413..4344944
• Replicon: chromosome
• Accession: NC_010172
• Organism: Methylobacterium extorquens PA1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: MEXT_RS19625
• Protein ID: WP_003605878.1
• Coordinates: 4344663..4344944 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C5B2A4
• Locus tag: MEXT_RS19620
• Protein ID: WP_003605879.1
• Coordinates: 4344413..4344676 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEXT_RS19600	4340453..4341079	+	627	WP_042508980.1	bifunctional nicotinamidase/pyrazinamidase	-
MEXT_RS19605	4341250..4342107	-	858	WP_012255092.1	aminotransferase class IV	-
MEXT_RS19610	4342094..4342723	-	630	WP_012255093.1	aminodeoxychorismate/anthranilate synthase component II	-
MEXT_RS19615	4342720..4344120	-	1401	WP_042508981.1	aminodeoxychorismate synthase component I	-
MEXT_RS19620	4344413..4344676	+	264	WP_003605879.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MEXT_RS19625	4344663..4344944	+	282	WP_003605878.1	type II toxin-antitoxin system YafQ family toxin	Toxin
MEXT_RS19630	4345061..4346407	+	1347	WP_012255095.1	30S ribosomal protein S12 methylthiotransferase RimO	-
MEXT_RS19635	4346504..4346689	+	186	WP_003605874.1	hypothetical protein	-
MEXT_RS19640	4346696..4347118	+	423	WP_012255096.1	DoxX family protein	-
MEXT_RS26540	4347207..4347398	+	192	WP_190274566.1	hypothetical protein	-
MEXT_RS19645	4347484..4348314	-	831	WP_012255097.1	nucleoside triphosphate pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-83)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10664.24 Da
• Isoelectric Point: 10.2159

>T22611 WP_003605878.1 NC_010172:4344663-4344944 [Methylorubrum extorquens PA1]
MRTIERTTQFKRDYKREMKGRHRASLQADLTAVLTELAADRPLAARLRDHALTGNWADHRDCHVKPDLVLIYRLAGTETL
QLVRLGSHAELGF

Nucleotide

• Length: 282 bp

>T22611 NC_010172:4344663-4344944 [Methylorubrum extorquens PA1]
ATGCGGACGATTGAGCGAACCACGCAGTTCAAGCGTGACTACAAGCGGGAGATGAAGGGCAGGCACCGGGCGAGCCTTCA
AGCCGACCTGACGGCGGTGCTGACCGAGTTGGCCGCGGATCGCCCCCTCGCGGCGCGCCTGCGTGACCACGCCCTGACCG
GCAACTGGGCCGACCATCGGGACTGCCACGTGAAGCCGGATCTGGTGCTGATCTATCGGCTTGCAGGAACGGAAACGCTC
CAATTGGTGCGCCTCGGCTCGCACGCCGAACTGGGCTTCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9356.81 Da
• Isoelectric Point: 4.4230

>AT22611 WP_003605879.1 NC_010172:4344413-4344676 [Methylorubrum extorquens PA1]
MPTKSLIQARIDADTKEEATAVLKAMGLTVSDAVRMMLVRVAREKALPFEPLVPNETTIAAIREARSGNLPGFTSVEALM
ADLDADD

Nucleotide

• Length: 264 bp

>AT22611 NC_010172:4344413-4344676 [Methylorubrum extorquens PA1]
ATGCCGACCAAGTCGCTGATCCAGGCGCGGATCGATGCCGACACCAAGGAAGAGGCCACAGCCGTTCTCAAGGCCATGGG
GCTGACCGTCTCCGATGCGGTTCGCATGATGCTCGTGCGCGTCGCGCGCGAGAAGGCGCTTCCCTTCGAGCCTCTGGTTC
CCAACGAAACGACCATCGCAGCCATCCGTGAGGCGCGGTCCGGTAACCTTCCGGGCTTCACCAGCGTCGAGGCGCTGATG
GCCGATCTCGATGCGGACGATTGA

Structures

Antitoxin

• AlphaFold DB: C5B2A4