Detailed information of TA system TA022609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 382921..383503
• Replicon: chromosome
• Accession: NC_010172
• Organism: Methylobacterium extorquens PA1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: MEXT_RS01655
• Protein ID: WP_012252142.1
• Coordinates: 383228..383503 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: MEXT_RS01650
• Protein ID: WP_012252141.1
• Coordinates: 382921..383238 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEXT_RS01635	378193..379167	-	975	WP_012252139.1	aliphatic sulfonate ABC transporter substrate-binding protein	-
MEXT_RS01640	379327..382113	+	2787	WP_003600762.1	[protein-PII] uridylyltransferase	-
MEXT_RS01645	382205..382813	+	609	WP_012252140.1	nucleotide exchange factor GrpE	-
MEXT_RS01650	382921..383238	-	318	WP_012252141.1	BrnA antitoxin family protein	Antitoxin
MEXT_RS01655	383228..383503	-	276	WP_012252142.1	BrnT family toxin	Toxin
MEXT_RS01660	383595..384452	-	858	WP_012252143.1	prephenate dehydratase	-
MEXT_RS01665	384469..385212	-	744	WP_003600755.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
MEXT_RS24865	385520..386437	+	918	WP_012252144.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-84)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-100)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10017.51 Da
• Isoelectric Point: 7.0130

>T22609 WP_012252142.1 NC_010172:c383503-383228 [Methylorubrum extorquens PA1]
MLIVWDEPKRLTNLQKHGLDFADFEAGFDFETALVAVAQPSAAGSARMKIIGEFDGQTVVAAIIAPLGREAISLISLRRA
SRSERRLYDAR

Nucleotide

• Length: 276 bp

>T22609 NC_010172:c383503-383228 [Methylorubrum extorquens PA1]
ATGTTGATTGTCTGGGACGAGCCCAAGCGCCTGACCAACCTGCAAAAGCACGGTCTCGACTTCGCGGATTTCGAGGCGGG
GTTCGACTTCGAGACCGCTCTTGTCGCGGTTGCCCAACCGAGCGCCGCTGGCTCAGCCCGAATGAAGATCATCGGCGAGT
TCGACGGACAGACGGTGGTGGCAGCGATCATCGCTCCCTTGGGACGGGAAGCGATCTCCCTCATCAGCCTGCGCCGAGCC
AGCCGAAGCGAACGGAGGCTCTACGATGCCCGCTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11616.97 Da
• Isoelectric Point: 4.6461

>AT22609 WP_012252141.1 NC_010172:c383238-382921 [Methylorubrum extorquens PA1]
MPADKPDRTLTTNSEEAGIQAGIATDPDNPEWTEADFQSAVPFEQAFPVLAEKLRRTRGPQSAPTKQLVSLRLDQDVLLR
FRATGPGWQSRMNEALRRAAADLPD

Nucleotide

• Length: 318 bp

>AT22609 NC_010172:c383238-382921 [Methylorubrum extorquens PA1]
ATGCCCGCTGACAAGCCCGATCGGACATTGACCACGAACTCGGAGGAGGCCGGGATTCAAGCCGGCATCGCGACCGATCC
CGACAATCCGGAATGGACCGAAGCGGACTTTCAAAGCGCCGTGCCCTTCGAACAGGCCTTTCCCGTCCTCGCAGAGAAAC
TGCGCCGCACCCGCGGCCCCCAGAGCGCTCCGACCAAACAGCTCGTTAGCTTGAGGCTCGATCAGGACGTCCTGCTTCGT
TTCAGGGCGACCGGTCCGGGCTGGCAGTCTCGGATGAACGAAGCCCTGCGCCGGGCCGCGGCCGATCTCCCCGACTGA