Detailed information of TA system TA226088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2588..3172
• Replicon: plasmid pAP2044-2
• Accession: NZ_CP087718
• Organism: Acinetobacter pittii strain AP2044

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: LPS07_RS20325
• Protein ID: WP_000286964.1
• Coordinates: 2588..2890 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LPS07_RS20330
• Protein ID: WP_000985609.1
• Coordinates: 2891..3172 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPS07_RS20305 (LPS07_20305)	482..679	-	198	Protein_1	IS30 family transposase	-
LPS07_RS20310 (LPS07_20310)	1232..1519	+	288	WP_000438825.1	BrnT family toxin	-
LPS07_RS20315 (LPS07_20315)	1506..1820	+	315	WP_230445356.1	BrnA antitoxin family protein	-
LPS07_RS20320 (LPS07_20320)	1909..2439	-	531	WP_000172759.1	hypothetical protein	-
LPS07_RS20325 (LPS07_20325)	2588..2890	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LPS07_RS20330 (LPS07_20330)	2891..3172	+	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
LPS07_RS20335 (LPS07_20335)	3252..3671	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
LPS07_RS20340 (LPS07_20340)	3811..4131	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LPS07_RS20345 (LPS07_20345)	4124..4396	+	273	WP_000369781.1	NadS family protein	-
LPS07_RS20350 (LPS07_20350)	4889..6364	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
LPS07_RS20355 (LPS07_20355)	6420..7304	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
LPS07_RS20360 (LPS07_20360)	7494..8105	-	612	WP_015060246.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / tet(39) / cmlB1 / sul2 / aph(3'')-Ib / aph(6)-Id	-	1..43577	43577
	-	inside	IScluster/Tn	msr(E) / mph(E) / tet(39) / cmlB1	-	482..13216	12734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T226088 WP_000286964.1 NZ_CP087718:2588-2890 [Acinetobacter pittii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T226088 NZ_CP121816:c902556-901237 [Aeromonas veronii]
ATGGCCAATCTGGCTGTCTACATGAACGGCTATCGGGTAGGCACATTCACCAAAACTACCAGCGGCGCCCATCAATTTCA
GTACCGTGAATCATGGCTAGCACAACCGGGCAGCCGTCCGATCTCACTCTCCATGCCATTGCGCCATCAGCCCTACCGGG
GCGATGAAGCCTATAACTTCTTCGATAACCTGCTGCCAGATAATATGGAGGTGAGGAACAGGGTTGTAGCACGCTATCAA
GCGGCATCAACTCAGCCCTTCGATCTGCTCAGTTGCATTGGCCAGGATAGCGTAGGGGCATTACAGCTGGTTACCGAAGG
GCACGATGTTCCAGATGTCAGGCGAATCGACTACAAGGCACTTTCCGATGACGAGCTCGAGCAGATCCTGACCAGCTACA
AATCAGGAATCCCTTTGGGGATGGTCAGGGAGCAAGAGGAGTTCCGGATCTCTATCGCCGGGGCGCAAGAAAAGACTGCG
CTGCTCTATCTCGATGACCGTTGGTACTTGCCACTCAACGCAACGCCGACAACCCACATTATCAAACTCCCTATCGGCAA
GATTGAAAGCCACTCCTACTCGATAGACCTCTCCCAGAGCGTAGAAAACGAATATCTCTGCACTCTGATTGCCAAGGAGT
TTGGTCTACCCGTACCTCACTGCTTCATGATGCAGGTTGGCCAGGTCAAAGCACTGGCTGTGGAACGCTTCGACAGACGT
TACGCTGCCGATCGCAGTTGGATCATGCGCCTTCCCCAAGAAGATTTCTGTCAGGTATTGAATGTTCCGTCAGCACGGAA
ATACGAGAACCATGGAGGAACGGGGATCACTGACATCATGAAAACCTTGCTGGGCTCTGCGACACCAGAACAGGATCGCT
ACCTATTCATGAAATCTCAGGTCCTGTTCTGGCTGCTGGCGGCCACCGATGGCCATGCAAAAAACTTCTCGGTCTTTATT
GAGCCGGAAGGGCGTTTTCGCCTGACCCCTTTTTACGACATCCTATCTGTCTATCCAGTATTTGGCGGCCGAGGCCTCAA
CCGAAGGGACGCCAAGCTCGCTATGGGCCTAGCAAGCAGCAAGGGAAAGAAGTATGCCATTGAGCAGATATTCCCGCGTC
ATTTCTTCCAAACGGCGAAAGCTGTGGGATTTGAGCGTTCTGCTATGGAAGAGATCCTGACAGAACTGGCCAACTCCGTT
GATGATGTTATTGAGCGCGTTACCCAGCAATTGCCAGCAGACTTCCCCGATACCATCAGCAGCACCATTCTAGAGGGCCT
AAAAGCCCGCTCAGCCAGGCTGATAAAGGGTTGGGATTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT226088 WP_000985609.1 NZ_CP087718:2891-3172 [Acinetobacter pittii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT226088 NZ_CP121816:c902810-902556 [Aeromonas veronii]
ATGAAAGTAACCAGCGCCAAGCAGCTGAGCGCCTGTATCAAGGATGCCAGGCTTTCTCAAAAGCTCTCTCAAGGCAAAGT
GGCCGAGAAAGTGGGGATTAGGCAAGACACAGTCTCCAGCTTCGAGCTCAATCCTGAATCCACCAAGCTGGAGACCCTGT
TCAAGATCCTCGCGGCACTCGAATTGTCACTGGACATCAAGCCCCGCAATGAGTCATCCGATAGTCCGGCATCGGGCTGG
AAGGAGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94