Detailed information of TA system TA226087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1232..1820
• Replicon: plasmid pAP2044-2
• Accession: NZ_CP087718
• Organism: Acinetobacter pittii strain AP2044

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8SM64
• Locus tag: LPS07_RS20310
• Protein ID: WP_000438825.1
• Coordinates: 1232..1519 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: LPS07_RS20315
• Protein ID: WP_230445356.1
• Coordinates: 1506..1820 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPS07_RS20305 (LPS07_20305)	482..679	-	198	Protein_1	IS30 family transposase	-
LPS07_RS20310 (LPS07_20310)	1232..1519	+	288	WP_000438825.1	BrnT family toxin	Toxin
LPS07_RS20315 (LPS07_20315)	1506..1820	+	315	WP_230445356.1	BrnA antitoxin family protein	Antitoxin
LPS07_RS20320 (LPS07_20320)	1909..2439	-	531	WP_000172759.1	hypothetical protein	-
LPS07_RS20325 (LPS07_20325)	2588..2890	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LPS07_RS20330 (LPS07_20330)	2891..3172	+	282	WP_000985609.1	putative addiction module antidote protein	-
LPS07_RS20335 (LPS07_20335)	3252..3671	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
LPS07_RS20340 (LPS07_20340)	3811..4131	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LPS07_RS20345 (LPS07_20345)	4124..4396	+	273	WP_000369781.1	NadS family protein	-
LPS07_RS20350 (LPS07_20350)	4889..6364	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / tet(39) / cmlB1 / sul2 / aph(3'')-Ib / aph(6)-Id	-	1..43577	43577
	-	inside	IScluster/Tn	msr(E) / mph(E) / tet(39) / cmlB1	-	482..13216	12734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11304.70 Da
• Isoelectric Point: 5.6205

>T226087 WP_000438825.1 NZ_CP087718:1232-1519 [Acinetobacter pittii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T226087 NZ_CP121802:2468961-2469614 [Aeromonas dhakensis]
ATGGCCTATGACTATAGCCAGAGCCTGAATCCGGAGAAGGCGCTGATATTCCGGATCGTGCATCGGGATAACCTGGAATG
GATCCTGACCCATGGCCTGTGGGCGGGGAATGGTCAGCAGCAAAACCCGCACTGGGTGCATATCGGGTTGCCGGAGCTGA
TTGCCAAGCGCGCGCGTCATCCGGTGCCGCTGGTGCCTTACGGCGTGTTGAATGACTATGTGCCCTTCTATTTCACGCCG
TTTTCTCCCATGCTCAGCAATATTCACTCGGGGCGCGGTGTTCCCCAGCGTGAAAATGATGAAATCCTGATCCTGGTTTC
CAGCCTGCATCGCATGGCTGAACTGGGGCTACCGTTTGTGTTTACCGATAGCCATGCCTATTACCAGTGGGCCAATTTCT
ATGTCGATCTTGGCGATCTGGAGCGGATTGACTGGCCACTGTTGCAGCGCCGTGATTTCAAGCGTGATGCGGAAGATTTG
AGCAAGCTGGAGCGCTATCAGGCCGAGGCCTTGATCCATCAACATTGCCCCATCGAGGCATTGCTGGGTGTGGTGTGTTA
CAACGAAGAGATAAAGAACCGCGTGGAACAATGCATGAATCGGGTCGGGGTCGCGCGCCCCGTCTATGTCCGTGATGGGT
GGTATTTCAGATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12242.94 Da
• Isoelectric Point: 8.4628

>AT226087 WP_230445356.1 NZ_CP087718:1506-1820 [Acinetobacter pittii]
MSMVRYSRKELNEKFVEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT226087 NZ_CP121802:2469611-2470675 [Aeromonas dhakensis]
ATGATTCGATATACAACAGGCAATCTGCTGGATGCTCCGGTGGAAGCGCTGGTGAATACCGTCAACACCGTCGGTGTCAT
GGGCAAGGGGATCGCCTTGATGTTCAAGGAGCGGTTCCCGGCCAATATGCGTGCCTATGCCGAGGCGTGCAAGCAGGGTG
ACGTCGTCACCGGCAAGATGTTTATTACCCAGACGGGTGAGCTGCTAGGGCCGCGCTGGATCGTCAACTTTCCGACCAAA
CAGCACTGGCGCAATCCGGCACAGATGGCGTGGATCCAGGAGGGTTTGCAGGATCTGCGGGCCTGGTTACAGGCGCAGCA
GGTCTCTTCGATTGCCATTCCGCCGCTGGGGGCTGGCAACGGTGGTCTGCCTTGGCCAGAGGTCAAACAGGCGATTGAAA
CGGCACTGGGCGATCTGGATATCGATATCCTGCTCTATGAGCCCAGTAGCCAATACCAAAATGTGGCCAAGCGGGGCGGG
GTGGCGGAGCTGACTCCGGCTCGGGCCTTGATTGCTGAACTGGTACGCCGCTACTGGTTTTTAGGAATGGAGTGCAGCTT
GCTGGAGATCCAGAAACTGGCCTGGTTTCTGGCTCGGGTCATTGGCCGTAACCCGCTGGGGGATCCGCTGCAGTTGCAGT
TTAAGGCCCATCGTTATGGCCCTTATGCCAACAATCTGACCCATCTGCTCAATGCGATGGACGGCAGTTATCTGCAGTCC
GACAAGCGCATTCCGGATAGCAAGCCGTTTGATGTCATCTGGTTTAATGAGGCCAAAAAACAAGAGCTTGCCGTTTATCT
CCAGACCGAGGCCAAAGCATTTTTACCGATACTGGAGCAGACGACCGCGTTAATCGATGGTTTTGAGTCGCCCTATGGCA
TGGAGTTATTGGCCACGGTCGATTGGTTGATCGCCGAAGAGGGCTGTGCGCCGACGCTGAGCGCGCTGCGCGAGGGGTTG
CACCGCTGGCCTGCGGGAACCCCGTGGGGTGAGCGTAAGGCGCGGCTGTTTGATGATCGCAGCCTGCAGATCGCCTTGAC
CCGTTTGCAGCAGGGATTGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TVQ4