Detailed information of TA system TA226082

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 2907180..2907979
• Replicon: chromosome
• Accession: NZ_CP087711
• Organism: Plesiomonas shigelloides strain 7A

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LPH35_RS12865
• Protein ID: WP_230432176.1
• Coordinates: 2907446..2907979 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8AQZ3
• Locus tag: LPH35_RS12860
• Protein ID: WP_010863417.1
• Coordinates: 2907180..2907449 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPH35_RS12845	2903382..2904182	-	801	WP_172534859.1	class III extradiol ring-cleavage dioxygenase	-
LPH35_RS12850	2904633..2904965	+	333	WP_036769710.1	50S ribosome-binding protein YggL	-
LPH35_RS12855	2905856..2906770	-	915	WP_230432175.1	hypothetical protein	-
LPH35_RS12860	2907180..2907449	+	270	WP_010863417.1	DUF1778 domain-containing protein	Antitoxin
LPH35_RS12865	2907446..2907979	+	534	WP_230432176.1	GNAT family N-acetyltransferase	Toxin
LPH35_RS12870	2908735..2909196	+	462	WP_178088746.1	DUF2559 family protein	-
LPH35_RS12875	2909189..2909791	+	603	WP_230432177.1	putative adenosine monophosphate-protein transferase Fic	-
LPH35_RS12880	2910037..2910249	+	213	WP_230432178.1	hypothetical protein	-
LPH35_RS12885	2911224..2911913	+	690	WP_230432179.1	protein N-lysine methyltransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2834964..2917017	82053
	-	inside	Integron	-	-	2902642..2923405	20763

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (95-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-81)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20280.70 Da
• Isoelectric Point: 8.8293

>T226082 WP_230432176.1 NZ_CP087711:2907446-2907979 [Plesiomonas shigelloides]
VSWSDKFVELDKSKHDRESFYCGESELDTFIQKFALKHMSAGISRTMVLPAKPVLPNNKLPISAFYTIAPTSICRDTLPE
LLAKKLPRYPIPVFLLAQLSVHSMYQGCGLGKVALIKALQYLWQVNSYIRAYAVVVDCLNENAESFYLKYGFEYLCEHNG
RKRLFLPMKTIEQLFNS

Nucleotide

• Length: 534 bp

>T226082 NZ_CP121798:4271795-4272220 [Aeromonas hydrophila]
ATGTCGATAGCACTCGTGCGAAGAGGCACGCTGCTGATCCCATCGGGCGCAACCAATCATCTGTTTTTTGTCTGTAACGA
CCCCATTCTTTACCCCAGAAACACCAAAGCAAGCTTTCTTGCGGTCAATATCAGCAGCATCGCCCCCAACATCGAGCATG
ATCGGACTTGTGTGCTTGATGTGGGGGATCACCCTTTCGTGCACCACCCGAGTTTTGTCTTTTACCGCAAAGCCGAGATT
TTCGGTGCAGCAACGGTCGGCGAACAAATCGCGGCTGGTGACATTCAAACCCACGCCATCTGTGAAGAGCCAACCTTTGC
ACGCATCTTGGCCGGGTTTGACGTGTCACCCCACGTCACCCCATTGATCCGCAACTTCTATCGTAAATACTGCCTTACCC
AGCCTGAGCCGTCCTGCGCTCCCTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9882.34 Da
• Isoelectric Point: 4.9059

>AT226082 WP_010863417.1 NZ_CP087711:2907180-2907449 [Plesiomonas shigelloides]
MATARLDLRIDEEIKAKAEKASALLGLKSLTEYVVRLMDENSTKVIAEHESITVSNDIFDQFIEACDKAKAPNEALKDAL
AFTKEKGIR

Nucleotide

• Length: 270 bp

>AT226082 NZ_CP121798:4271238-4271795 [Aeromonas hydrophila]
ATGTTTAACGAAGAGCGTGTGGCACAAATGGCCGCCTTCCTGCTTTCCAAGCGGGGCGGACGAATGGCATACCTCAAACT
GATGAAACTGCTCTACCTGAGTGATCGGGAGTCCATGAGCCGTTTCGGTGAATCCATCAGCGGAGACCTCATGGTGTCCA
TGCCCCATGGGCCCGTGCTGTCACAGACGTTGAATCTGATCCGCGGTGGCGGTGAGCATGCTGACGGCTGGAATGAGTGG
ATCTCGGGCGAAGCGAACTACGAGGTGGCAATCCGTCGCAATGTGACCGGGCGGGATGATTTCGACTTGCTCAGCGATGT
GGATTTGGAAATCCTGACCAAGGTATGGCACGAGTTTGGGCACATGACTCGATACCAAATCCGTGACTACACCCATGACC
ATTGCCCGGAGTGGCAAGACCCCAGAGGCAGTGCTTACCCCATCAAGGAAGAAGACCTGTTTTTGGCAGTCGGCAAGTCG
CCGGAAGTAGCTGGCGCCATGGCGCAACATCTGCGCGAACAACGCCAACTCGATGAAATCACCAGCAGGTTGCGCTAA

Structures

Antitoxin

• AlphaFold DB: R8AQZ3