226081

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 155512..156248 Replicon plasmid pYUYZH2487-2
Accession NZ_CP087708
Organism Klebsiella pneumoniae strain YZH2487

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LO768_RS27930 Protein ID WP_003026803.1
Coordinates 155512..155994 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LO768_RS27935 Protein ID WP_003026799.1
Coordinates 155982..156248 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO768_RS27910 (LO768_27915) 151231..151626 - 396 WP_004143398.1 helix-turn-helix domain-containing protein -
LO768_RS27915 (LO768_27920) 151675..153021 - 1347 WP_077253535.1 ISNCY family transposase -
LO768_RS27920 (LO768_27925) 153247..153879 + 633 WP_001567369.1 hypothetical protein -
LO768_RS27925 (LO768_27930) 153908..155311 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
LO768_RS27930 (LO768_27935) 155512..155994 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LO768_RS27935 (LO768_27940) 155982..156248 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LO768_RS27940 (LO768_27945) 156424..156678 - 255 WP_004152108.1 hypothetical protein -
LO768_RS27945 (LO768_27950) 156754..157011 - 258 WP_004152107.1 hypothetical protein -
LO768_RS27950 (LO768_27955) 157060..157263 - 204 WP_004152106.1 HHA domain-containing protein -
LO768_RS27955 (LO768_27960) 157297..157665 - 369 WP_004152105.1 hypothetical protein -
LO768_RS27960 (LO768_27965) 157709..158203 - 495 WP_004152104.1 DNA-binding protein -
LO768_RS27965 (LO768_27970) 158234..158809 - 576 WP_004152103.1 hypothetical protein -
LO768_RS27970 (LO768_27975) 158797..159066 - 270 WP_004152102.1 hypothetical protein -
LO768_RS27975 (LO768_27980) 159424..159774 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
LO768_RS27980 (LO768_27985) 159824..160186 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / tet(A) / qnrB1 / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 - 1..210296 210296


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T226081 WP_003026803.1 NZ_CP087708:c155994-155512 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T226081 NZ_CP121796:43846-44043 [Aeromonas hydrophila]
GTGAACGAGGAGGCTACTTTGACAAGCAGTGCGCTAATCAAGCTACTAGAAGAAAATGGATGGGTCTGGGAGAGGACGAA
AGGCAGTCATCACCAGTATAAAAAGGAGGGATTTTCTTTTGTTATCACAGTCCCTCACCCAACCAAGGACATTAAGATCG
GAACGCTGAAAAAAAATAATGAAGCAAGCCGGTCTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT226081 WP_003026799.1 NZ_CP087708:c156248-155982 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT226081 NZ_CP121796:44189-44602 [Aeromonas hydrophila]
ATGTATTACTACCCTGCATATATCCACCAAGATGAAGACGGCTCTGCGAGTGGGTTCTTTCCTGGCGTTGACGGCTGTTT
CTTTGCTGGCAACACTCTGGAAGAGTGTATGGAAGATGCCAGAGGGGCCCTGGACGCACATTTCGAATTTATGGCCGACG
AGGGGTTACAAATCCCTGCGGCAACCAAGGTTTCAGAGCACTCCAAGGATGAGGAGTGTAAAGATGGATTCTGGGTTATG
GTCGAGATTGACACCTCAAAATTTGAGGGTGACATCAAGAGAATTAATATAACCCTTCCAGAAAACTTAATTGGAAAAAT
TGACAATAGAGTCTCAAGCGAAGGTAAAAGATACGCGAGTAGATCAGCTTTTATTGCTGCAGCTGCTAGAGAGCTATTGC
AAAGACACGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References