Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 8175..8911 Replicon plasmid pYUYZH2487-1
Accession NZ_CP087707
Organism Klebsiella pneumoniae strain YZH2487

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LO768_RS25970 Protein ID WP_003026803.1
Coordinates 8429..8911 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LO768_RS25965 Protein ID WP_003026799.1
Coordinates 8175..8441 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO768_RS25920 (LO768_25925) 3511..4200 + 690 WP_004193995.1 hypothetical protein -
LO768_RS25925 (LO768_25930) 4232..4921 - 690 WP_020315560.1 RES family NAD+ phosphorylase -
LO768_RS25930 (LO768_25935) 5358..5627 + 270 WP_004152102.1 hypothetical protein -
LO768_RS25935 (LO768_25940) 5615..6190 + 576 WP_004152103.1 hypothetical protein -
LO768_RS25940 (LO768_25945) 6221..6715 + 495 WP_004152104.1 DNA-binding protein -
LO768_RS25945 (LO768_25950) 6759..7127 + 369 WP_230444826.1 hypothetical protein -
LO768_RS25950 (LO768_25955) 7161..7364 + 204 WP_004152106.1 HHA domain-containing protein -
LO768_RS25955 (LO768_25960) 7413..7670 + 258 WP_004152107.1 hypothetical protein -
LO768_RS25960 (LO768_25965) 7745..7999 + 255 WP_004152108.1 hypothetical protein -
LO768_RS25965 (LO768_25970) 8175..8441 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LO768_RS25970 (LO768_25975) 8429..8911 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LO768_RS25975 (LO768_25980) 9119..10465 + 1347 WP_077253535.1 ISNCY family transposase -
LO768_RS25980 (LO768_25985) 10514..10909 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -
LO768_RS25985 (LO768_25990) 11057..12222 - 1166 Protein_17 IS3 family transposase -
LO768_RS25990 (LO768_25995) 12399..13361 - 963 WP_004152113.1 zinc metalloprotease HtpX -
LO768_RS25995 (LO768_26000) 13348..13836 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 - 1..217602 217602


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T226080 WP_003026803.1 NZ_CP087707:8429-8911 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T226080 NZ_CP087707:8429-8911 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT226080 WP_003026799.1 NZ_CP087707:8175-8441 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT226080 NZ_CP087707:8175-8441 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References